LOCUS       sequence-8315-ex        3953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Tessier PM, Lindquist S
  TITLE     Prion recognition elements govern nucleation, strain specificity and
            species barriers.
  JOURNAL   Nature. 2007 May 31. 447(7144):556-61.
  PUBMED    17495929
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 May 31. 447(7144):556-61."
COMMENT     Sequence Label: Sup35 ScNM A230C His7
FEATURES             Location/Qualifiers
     source          1..3953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             73..95
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             862..882
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(954..972)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      968..1015
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1262..1279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1433..2021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1513..1532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2192..2983,2984..3052))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2815..2834
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3053..3157)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3225..3243
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3281..3303)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3403..3422
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3440..3953)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3635..3656)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3847..3866
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggggatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
       61 tctagaaata attttgttta actttaagaa ggagatatac atatgtcgga ttcaaaccaa
      121 ggcaacaatc agcaaaacta ccagcaatac agccagaacg gtaaccaaca acaaggtaac
      181 aacagatacc aaggttatca agcttacaat tgccaagccc aacctgcagg tgggtactac
      241 caaaattacc aaggttattc tgggtaccaa caaggtggct atcaacagta caatcccgac
      301 gccggttacc agcaacagta taatcctcaa ggaggctatc aacagtacaa tcctcaaggc
      361 ggttatcagc agcaattcaa tccacaaggt ggccgtggaa attacaaaaa cttcaactac
      421 aataacaatt tgcaaggata tcaagctggt ttccaaccac agtctcaagg tatgtctttg
      481 aacgactttc aaaagcaaca aaagcaggcc gctcccaaac caaagaagac tttgaagctt
      541 gtctccagtt ccggtatcaa gttggccaat gctaccaaga aggttggcac aaaacctgcc
      601 gaatctgata agaaagagga agagaagtct gctgaaacca aagaaccaac taaagagcca
      661 acaaaggtcg aagaaccagt taaaaaggag gagaaaccag tccagactga agaaaagacg
      721 gaggaaaaat cggaacttcc aaaggtagaa gaccttaaaa tctctgaatc aacacataat
      781 accaacaatg ccaatgttac cagtgctgat gccttgatca aggaacagga agaagaagtg
      841 gatgacgaag ttgttaacga tcatcatcac catcaccacc attaagaatt catcgatgat
      901 atcagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      961 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     1021 aggaggaact atatccggat cggatatcac aggacgggtg tggtcgccat gatcgcgtag
     1081 tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac
     1141 agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagaggctgc
     1201 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1261 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1321 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1381 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1441 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1501 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1561 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1621 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1681 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1741 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1801 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1861 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1921 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1981 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2041 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2101 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2161 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2221 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2281 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2341 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2401 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2461 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2521 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2581 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2641 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2701 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2761 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2821 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2881 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2941 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3001 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3061 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3121 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3181 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3241 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     3301 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     3361 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     3421 tactgagagt gcagtaccca ccccggttga taatcagaaa agccccaaaa acaggaagat
     3481 tgtataagca aatatttaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
     3541 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3601 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3661 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3721 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3781 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3841 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3901 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgt
//
