LOCUS       sequence-83356-e        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ectopic expression of Lyr with KDEL tag for CTAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Tape CJ, Norrie IC, Worboys JD, Lim L, Lauffenburger DA, Jorgensen C
  TITLE     Cell-specific labeling enzymes for analysis of cell-cell 
            communication in continuous co-culture.
  JOURNAL   Mol Cell Proteomics. 2014 May 12.
  PUBMED    24820872
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2014 May 12."
COMMENT     Sequence Label: Lyr_M37_KDEL
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 gagctcggat ccactagtcc agtgtggtgg aattcgccac catggccatc gtgaacaaca
      181 gctggatcga gatcagcaga agcgccctgg acttcaacgt gaagaaggtg cagagcctgc
      241 tgggcaagca gagcagcctg tgtgctgtgc tgaagggcga cgcctacggc cacgatctgt
      301 ctctggtggc ccccatcatg atcgagaaca acgtgaagtg catcggcgtg accaacaacc
      361 aggaactgaa agaagtccgg gacctgggct tcaagggcag actgatgaga gtgcggaacg
      421 ccaccgagca ggaaatggcc caggccacca actacaacgt ggaagaactg atcggcgacc
      481 tggacatggc caagagactg gacgctatcg ccaagcagca gaacaaagtg atccccatcc
      541 acctggctct gaacagcggc ggcatgagca gaaacggcct ggaagtggac aacaagagcg
      601 gcctggaaaa ggccaagcag atcagccagc tggccaacct gaaggtcgtg ggcatcatga
      661 gccactaccc cgaagaggac gccaacaaag tgcgcgagga cctggccaga ttcaagcagc
      721 agtcccagca ggtcctggaa gtgatgggcc tggaaagaaa caacgtgacc ctgcacatgg
      781 ctaacacctt cgccaccatc accgtgcccg agagctggct ggacatggtc cgagtgggcg
      841 gcatcttcta cggcgacaca atcgccagca ccgactacaa gagagtgatg accttcaaga
      901 gcaatatcgc cagcatcaac tactacccca agggcaacac cgtgggctac gacagaacct
      961 acaccctgaa gagggacagc gtgctggcca acatccctgt gggctacgcc gacggctaca
     1021 gaagagtgtt cagcaacgcc ggccacgccc tgatcgctgg acagagagtg cctgtcctgg
     1081 gcaagaccag catgaacacc gtgatcgtgg acatcaccag cctgaaca
//
