LOCUS       sequence-83437-e        4822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inhibition module. Constructed from the parent plasmids pLuxRI, 
            pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 
            lysozyme by inducing AHL and LuxR. Chloramphenicol resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Payne S, Li B, Cao Y, Schaeffer D, Ryser MD, You L
  TITLE     Temporal control of self-organized pattern formation without 
            morphogen gradients in bacteria.
  JOURNAL   Mol Syst Biol. 2013 Oct 8;9:697. doi: 10.1038/msb.2013.55.
  PUBMED    24104480
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-8"; volume: "9:697. doi"; pages: " 
            10.1038/msb.2013.55"
COMMENT     Sequence Label: pTuLys2CMR2
FEATURES             Location/Qualifiers
     source          1..4822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             139..849
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(284..302)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     552..571
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             886..1341
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKACPSFDLKRWWEKNELVTSDRG"
     promoter        1339..1372
                     /label=T7 Phi-3.8 promoter
                     /note="weak promoter for bacteriophage T7 RNA polymerase"
     promoter        1587..1689
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1690..2349
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1756..1775)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2370..2464
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2578..3123
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     3024..3043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3240..3257
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3292..3378)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             4802..4813
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tatagtgagt cgtattatca tgagtcacac tattgtatcg ctgggaatac aattacttaa
       61 cataagcacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta tagtcgaata
      121 aacgcaaggg aggttggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
      181 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      241 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      301 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      361 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      421 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      481 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      541 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      601 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
      661 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      721 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      781 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      841 tacaagtaaa agcttgacta gctagcggaa aggaggaaag aaataatggc tcgtgtacag
      901 tttaaacaac gtgaatctac tgacgcaatc tttgttcact gctcggctac caagccaagt
      961 cagaatgttg gtgtccgtga gattcgccag tggcacaaag agcagggttg gctcgatgtg
     1021 ggataccact ttatcatcaa gcgagacggt actgtggagg caggacgaga tgagatggct
     1081 gtaggctctc acgctaaggg ttacaaccac aactctatcg gcgtctgcct tgttggtggt
     1141 atcgacgata aaggtaagtt cgacgctaac tttacgccag cccaaatgca atcccttcgc
     1201 tcactgcttg tcacactgct ggctaagtac gaaggcgctg tgcttcgcgc ccatcatgag
     1261 gtggcgccga aggcttgccc ttcgttcgac cttaagcgtt ggtgggagaa gaacgaactg
     1321 gtcacttctg accgtggata attaattgaa ctcactaaag ggagaccaca gcggtttccc
     1381 tttgttcgca ttggaggtca aataatgcgc aagtcttata aacaattcta taaggctccg
     1441 aggaggcata tccaagtgtg ggaggcagcc aatgggccgg atccacagga cgggtgtggt
     1501 cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggac
     1561 gtcgatatct ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat
     1621 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     1681 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     1741 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     1801 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     1861 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg
     1921 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     1981 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     2041 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     2101 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     2161 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     2221 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat
     2281 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     2341 ggggcgtaat ttgatatcga gctcgcttgg actcctgttg atagatccag taatgacctc
     2401 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
     2461 gaatccaagc actagtaaca acttatatcg tatggggctg acttcaggtg ctacatttga
     2521 agagataaat tgcactgaaa tctagtaata ttttatctga ttaataagat gatcttcttg
     2581 agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg
     2641 cggtttttcg aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg
     2701 agcgcagtca ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac
     2761 taactcctct aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg
     2821 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt
     2881 cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa
     2941 tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga
     3001 gagcgcacga gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3061 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
     3121 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca
     3181 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta
     3241 gcgagtcagt gagcgaggaa gcggaatata tccctaggtc tagggcggcg gatttgtcct
     3301 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
     3361 agcctttcgt tttatttgat gcctctagat taattaatta agcggccgcg aagcttaaca
     3421 acattaattt aagactgctt ttttaaactg ttcattaata ggcatagaca atacaaccga
     3481 tttagtatca cctaatacat gaatttcttt gtctccaata cgatgacaag gaactttaat
     3541 acgctttaaa aatcgctcta ttgctgttga tgttactgtt acatattctg taataccttg
     3601 actaacagcg tgtttatata tagcttcaaa tagtttcatt gtaatttcac tagcagagtt
     3661 atttatcttt gagctatttt tacctacagc aaaacgactt aattcgacta tattaggatc
     3721 tttgggagca ctctgttgac caagcaattc aggaaaaaca cttttcagca tataatcacc
     3781 tgttgtaggt aataaacgcc agcatccact tacattttca gtatcatcac aagcataaat
     3841 atattctgca tttgagttat catactcatc tgattcaagg ttattttcta caactaagtc
     3901 ccactcaagt ctttgcttaa acacttgata acgaagactt agaatacctt tatactcctc
     3961 cgatggaatt gccaaaaaat ccgatttttt tatcattata gtcataccaa cctcccttgc
     4021 gtttattcgg ggatcccgta cttaattttt aaagtatggg caatcaattg ctcctgttaa
     4081 aattgcttta gaaatacttt ggcagcggtt tgttgtattg agtttcattt gcgcattggt
     4141 taaatggaaa gtgacagtac gctcactgca gcctaatatt tttgaaatat cccaagagct
     4201 ttttccttcg catgcccacg ctaaacattc tttttctctt ttggttaaat cgttgtttga
     4261 tttattattt gctatattta tttttcgata attatcaact agagaaggaa caattaatgg
     4321 tatgttcata cacgcatgta aaaataaact atctatatag ttgtcttttt ctgaatgtgc
     4381 aaaactaagc attccgaagc cattgttagc cgtatgaata gggaaactaa acccagtgat
     4441 aagacctgat gttttcgctt ctttaattac atttggagat tttttattta cagcattgtt
     4501 ttcaaatata ttccaattaa ttggtgaatg attggagtta gaataatcta ctataggatc
     4561 atattttatt aaattagcgt catcataata ttgcctccat tttttagggt aattatctag
     4621 aattgaaata tcagatttaa ccatagaatg aggataaatg atcgcgagta aataatattc
     4681 acaatgtacc attttagtca tatcagataa gcattgatta atatcattat tgcttctaca
     4741 agctttaatt ttattaatta ttctgtatgt gtcgtcggca tttatgtttt tcatgggtac
     4801 ctttctcctc tttaatgaat tc
//
