LOCUS       sequence-83452-e        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector for enhanced eGFP 
            expression/marking in primary B cells and B-cell lines .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Mock U, Thiele R, Uhde A, Fehse B, Horn S
  TITLE     Efficient lentiviral transduction and transgene expression in 
            primary human B cells.
  JOURNAL   Hum Gene Ther Methods. 2012 Dec;23(6):408-15. doi: 
            10.1089/hgtb.2012.160.
  PUBMED    23240650
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hgtb.2012";
            journalName: "Hum Gene Ther Methods"; date: "2012-12"; volume: "23";
            issue: "6"; pages: "408-15"
COMMENT     Sequence Label: LeGO-hEµMAR-G2
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..30
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     15..31
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(73..106)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gggcccgctc tagtcgaggt cgacggtatc gataagctcg cttcacgaga ttccagcagg
       61 tcgagggacc taataacttc gtatagcata cattatacga agttatatta agggttccaa
      121 gcttaagcgg ccgctgaaag accccacctg taggtttggc aagctagcag gattgtttat
      181 cttaggaggc atgcttactg ttaaaagaca ggatatgttt gaagtggctt ctgagaaaaa
      241 tggttaagaa aattatgact taaaaatgtg agagattttc aagtatatta atttttttaa
      301 ctgtccaagt atttgaaatt cttatcattt gattaacacc catgagtgat atgtgtctgg
      361 aattgaggcc aaagcaagct cagctaagaa atactagcac agtgctgtcg gccccgatgc
      421 gggactgcgt tttgaccatc ataaatcaag tttatttttt taattaattg agcgaagctg
      481 gaagcagatg atgaattaga gtcaagatgg ctgcatgggg gtctccggca cccacagcag
      541 gtggcaggaa gcaggtcacc gcgagagtct attttaggaa gcaaaaaaac acaattggta
      601 aatttatcac ttctggttgt gaagaggtgg ttttgcccag gcccagatct gaaagtgctc
      661 tactgagcaa aacaacacct ggacaatttg cgtttctaaa ataaggcgag gctgaccgaa
      721 actgaaaagg ctttttttaa ctatctgaat ttcatttcca atcttagctt atcaactgct
      781 agtttgtgca aacagcatat caacttctaa actgcattca tttttaaagt aagatgttta
      841 agaaattaaa cagtcttagg gagagtttat gactgtattc aaaaagtttt ttaaattagc
      901 ttgttatccc ttcatgtgat aactaatctc aaatactttt tcgatacctc agagcattat
      961 tttcataatg actgtgttca caatcttttt aggttaactc gttctctctt tgtgattaag
     1021 gagaaacact ttgatattct gatagagtgg ccttcattt
//
