LOCUS       sequence-83729-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  chromosomal tagging with stable GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Ko N, Nishihama R, Tully GH, Ostapenko D, Solomon MJ, Morgan DO, 
            Pringle JR
  TITLE     Identification of yeast IQGAP (Iqg1p) as an 
            anaphase-promoting-complex substrate and its role in 
            actomyosin-ring-independent cytokinesis.
  JOURNAL   Mol Biol Cell. 2007 Dec;18(12):5139-53. Epub 2007 Oct 17.
  PUBMED    17942599
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2007-12"; volume: "18(12)"; pages: "5139-53. Epub 
            2007 Oct 17"
COMMENT     Sequence Label: pFA6a-GFP (F64L, S65T, V163A)-kanMX6
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..746
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(63..91)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     686..706
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 acgctgcagg tcgacggatc cccgggttaa ttaacagtaa aggagaagaa cttttcactg
       61 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      121 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
      181 ctggaaaact acctgttcca tggccaacac ttgtcactac tctcacttat ggtgttcaat
      241 gcttttcaag atacccagat cacatgaaac agcatgactt tttcaagagt gccatgcccg
      301 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
      361 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
      421 ttaaagaaga tggaaacatt cttgggcaca aattggaata caactataac tcacacaatg
      481 tatacatcat ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attagacaca
      541 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
      601 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
      661 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
      721 cacatggcat ggatgaacta tacaaatagg gcgcgccact tctaaataag cgaatttctt
      781 atgatttatg atttttatta ttaaataagt tataaaaaaa at
//
