LOCUS       sequence-83756-e        2023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2023)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MTRA-c034
FEATURES             Location/Qualifiers
     source          1..2023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga aaaccctgcg ggatgagatc aatgccattc tgcagaagag gattatggtg
      121 ctggatggag ggatggggac catgatccag cgggagaagc taaacgaaga acacttccga
      181 ggtcaggaat ttaaagatca tgccaggccg ctgaaaggca acaatgacat tttaagtata
      241 actcagcctg atgtcattta ccaaatccat aaggaatact tgctggctgg ggcagatatc
      301 attgaaacaa atacttttag cagcactagt attgcccaag ctgactatgg ccttgaacac
      361 ttggcctacc ggatgaacat gtgctctgca ggagtggcca gaaaagctgc cgaggaggta
      421 actctccaga caggaattaa gaggtttgtg gcaggggctc tgggtccgac taataagaca
      481 ctctctgtgt ccccatctgt ggaaaggccg gattatagga acatcacatt tgatgagctt
      541 gttgaagcat accaagagca ggccaaagga cttctggatg gcggggttga tatcttactc
      601 attgaaacta tttttgatac tgccaatgcc aaggcagcct tgtttgcact ccaaaatctt
      661 tttgaggaga aatatgctcc ccggcctatc tttatttcag ggacgatcgt tgataaaagt
      721 gggcggactc tttccggaca gacaggagag ggatttgtca tcagcgtgtc tcatggagaa
      781 ccactctgca ttggattaaa ttgtgctttg ggtgcagctg aaatgagacc ttttattgaa
      841 ataattggaa aatgtacaac agcctatgtc ctctgttatc ccaatgcagg tcttcccaac
      901 acctttggtg actatgatga aacgccttct atgatggcca agcacctaaa ggattttgct
      961 atggatggct tggtcaatat agttggagga tgctgtgggt caacaccaga tcatatcagg
     1021 gaaattgctg aagctgtgaa aaattgtaag cctagagttc cacctgccac tgcttttgaa
     1081 ggacatatgt tactgtctgg tctagagccc ttcaggattg gaccgtacac caactttgtt
     1141 aacattggag agcgctgtaa tgttgcagga tcaaggaagt ttgctaaact catcatggca
     1201 ggaaactatg aagaagcctt gtgtgttgcc aaagtgcagg tggaaatggg agcccaggtg
     1261 ttggatgtca acatggatga tggcatgcta gatggtccaa gtgcaatgac cagattttgc
     1321 aacttaattg cttccgagcc agacatcgca aaggtacctt tgtgcatcga ctcctccaat
     1381 tttgctgtga ttgaagctgg gttaaagtgc tgccaaggga agtgcattgt caatagcatt
     1441 agtctgaagg aaggagagga cgacttcttg gagaaggcca ggaagattaa aaagtatgga
     1501 gctgctatgg tggtcatggc ttttgatgaa gaaggacagg caacagaaac agacacaaaa
     1561 atcagagtgt gcacccgggc ctaccatctg cttgtgaaaa aactgggctt taatccaaat
     1621 gacattattt ttgaccctaa tatcctaacc attgggactg gaatggagga acacaacttg
     1681 tatgccatta attttatcca tgcaacaaaa gtcattaaag aaacattacc tggagccaga
     1741 ataagtggag gtctttccaa cttgtccttc tccttccgag gaatggaagc cattcgagaa
     1801 gcaatgcatg gggttttcct ttaccatgca atcaagtctg gcatggacat ggggatagtg
     1861 aatgctggaa acctccctgt gtatgatgat atccataagg aacttctgca gctctgtgaa
     1921 gatctcatct ggaataaaga ccctgaggcc actgagaagc tcttacgtta tgcccagact
     1981 caaggcacag gagggtgaca gtaaaggtgg atacggatcc gaa
//
