LOCUS       sequence-83757-e        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GAKA-c028
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..33
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca tcaccatcat cacgatctgg gtaccgagaa cctgtacttc
       61 caatccatgc caggctccct gggcggtgct tccggccgcg accagagtga cttcgtgggg
      121 cagacggtgg aactgggcga gctgcggctg cgggtgcggc gggtcctggc cgaaggaggg
      181 tttgcatttg tgtatgaagc tcaagatgtg gggagtggca gagagtatgc attaaagagg
      241 ctattatcca atgaagagga aaagaacaga gccatcattc aagaagtttg cttcatgaaa
      301 aagctttccg gccacccgaa cattgtccag ttttgttctg cagcgtctat aggaaaagag
      361 gagtcagaca cggggcaggc tgagttcctc ttgctcacag agctctgtaa agggcagctg
      421 gtggaatttt tgaagaaaat ggaatctcga ggcccccttt cgtgcgacac ggttctgaag
      481 atcttctacc agacgtgccg cgccgtgcag cacatgcacc ggcagaagcc gcccatcatc
      541 cacagggacc tcaaggttga gaacttgttg cttagtaacc aagggaccat taagctgtgt
      601 gactttggca gtgccacgac catctcgcac taccctgact acagctggag cgcccagagg
      661 cgagccctgg tggaggaaga gatcacgagg aatacaacac caatgtatag aacaccagaa
      721 atcatagact tgtattccaa cttcccgatc ggcgagaagc aggatatctg ggccctgggc
      781 tgcatcttgt acctgctgtg cttccggcag cacccttttg aggatggagc gaaacttcga
      841 atagtcaatg ggaagtactc gatccccccg cacgacacgc agtacacggt cttccacagc
      901 ctcatccgcg ccatgctgca ggtgaacccg gaggagcggc tgtccatcgc cgaggtggtg
      961 caccagctgc aggagatcgc ggccgcccgc aacgtgaacc ccaagtctcc catcacagag
     1021 ctcctggagc agaatggagg ctacgggagc gccacactgt cccgagggcc accccctccc
     1081 gtgtgacagt aaaggtggat acggatcc
//
