LOCUS       sequence-83775-e        1269 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1269)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAT2AA-c001
FEATURES             Location/Qualifiers
     source          1..1269
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga acggacagct caacggcttc catgaggcgt tcatcgagga gggcacattc
      121 cttttcacct cagagtcggt cggggaaggc cacccagata agatttgtga ccaaatcagt
      181 gatgctgtcc ttgatgccca ccttcagcag gatcctgatg ccaaagtagc ttgtgaaact
      241 gttgctaaaa ctggaatgat ccttcttgct ggggaaatta catccagagc tgctgttgac
      301 taccagaaag tggttcgtga agctgttaaa cacattggat atgatgattc ttccaaaggt
      361 tttgactaca agacttgtaa cgtgctggta gccttggagc aacagtcacc agatattgct
      421 caaggtgttc atcttgacag aaatgaagaa gacattggtg ctggagacca gggcttaatg
      481 tttggctatg ccactgatga aactgaggag tgtatgcctt taaccattgt cttggcacac
      541 aagctaaatg ccaaactggc agaactacgc cgtaatggca ctttgccttg gttacgccct
      601 gattctaaaa ctcaagttac tgtgcagtat atgcaggatc gaggtgctgt gcttcccatc
      661 agagtccaca caattgttat atctgttcag catgatgaag aggtttgtct tgatgaaatg
      721 agggatgccc taaaggagaa agtcatcaaa gcagttgtgc ctgcgaaata ccttgatgag
      781 gatacaatct accacctaca gccaagtggc agatttgtta ttggtgggcc tcagggtgat
      841 gctggtttga ctggacgcaa aatcattgtg gacacttatg gcggttgggg tgctcatgga
      901 ggaggtgcct tttcaggaaa ggattatacc aaggtcgacc gttcagctgc ttatgctgct
      961 cgttgggtgg caaaatccct tgttaaagga ggtctgtgcc ggagggttct tgttcaggtc
     1021 tcttatgcta ttggagtttc tcatccatta tctatctcca ttttccatta tggtacctct
     1081 cagaagagtg agagagagct attagagatt gtgaagaaga atttcgatct ccgccctggg
     1141 gtcattgtca gggatctgga tctgaagaag ccaatttatc agaggactgc agcctatggc
     1201 cactttggta gggacagctt cccatgggaa gtgcccaaaa agcttaaata ttgacagtaa
     1261 aggtggata
//
