LOCUS       sequence-83781-e        1158 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1158)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ELAC1A-c005
FEATURES             Location/Qualifiers
     source          1..1158
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1087..1107
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1108..1125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1126..1149
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggatgtga cattcctggg gacgggtgca gcatacccat ctccaacccg gggtgcctct
       61 gctgtggtcc ttcggtgtga aggcgagtgc tggctctttg actgtgggga gggaacacag
      121 acacagctta tgaaaagcca acttaaagca gggagaatta ccaagatctt catcacacac
      181 cttcatggag accatttctt tggccttcct gggctcctct gcacaatcag cctgcagagt
      241 ggctccatgg tgtccaaaca gcctattgaa atctatggcc ctgtagggct tcgggacttt
      301 atctggcgaa ccatggaact ctctcacacg gagctggtct tccattatgt ggttcatgaa
      361 ctggttccta cagcagatca atgtcctgca gaagaactaa aagaatttgc gcatgtgaat
      421 agagcagaca gtcctcccaa agaggaacaa ggaagaacta tcctgttaga ctcagaagaa
      481 aactcatacc ttctgtttga tgatgaacaa tttgttgtaa aagcatttcg cctctttcac
      541 agaattccct catttgggtt ttcagtcgtg gaaaagaaac gcccaggtaa actcaatgca
      601 cagaaactta aagaccttgg tgttccacca ggtcctgcct atgggaagct gaaaaatgga
      661 atttctgttg ttctggaaaa tggggttaca atttctcccc aagatgtctt aaaaaagcct
      721 attgttggaa gaaaaatctg catattgggt gactgctctg gggttgtggg tgatggagga
      781 gtaaaactgt gctttgaagc agacctgttg atccacgaag caaccctgga tgatgcccag
      841 atggacaaag caaaggagca tggccacagc acaccacaga tggcagcaac atttgcaaag
      901 ttgtgccgtg caaagaggct ggttctgact cacttcagtc agaggtacaa accagttgcc
      961 ttggccagag aaggagaaac agatggcatt gcagaactaa aaaagcaagc tgaatcagtg
     1021 ttagatctcc aagaagtgac tctagcagaa gattttatgg tgataagcat tccaatcaag
     1081 aaagcagaga acctctactt ccaatcgcac catcatcacc accatgatta caaggatgac
     1141 gacgataagt gaggatcc
//
