LOCUS       sequence-83828-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RIG-I with a mutation to motif III in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Bamming D, Horvath CM
  TITLE     Regulation of signal transduction by enzymatically inactive 
            antiviral RNA helicase proteins MDA5, RIG-I, and LGP2.
  JOURNAL   J Biol Chem. 2009 Apr 10;284(15):9700-12. doi: 
            10.1074/jbc.M807365200. Epub 2009 Feb 11.
  PUBMED    19211564
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M807365200"; journalName: "J Biol Chem"; date: 
            "2009-04-10- 10"; volume: "284"; issue: "15"; pages: "9700-12"
COMMENT     Sequence Label: pEF-Bos-FLAG RIG-I MIII
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..42
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctagataag ccaccatgga ttataaggat gatgatgata aaggtgatta taaggatgat
       61 gatgataaag gtaccaccga gcagcgacgc agcctgcaag ccttccagga ttatatccgg
      121 aagaccctgg accctaccta catcctgagc tacatggccc cctggtttag ggaggaagag
      181 gtgcagtata ttcaggctga gaaaaacaac aagggcccaa tggaggctgc cacacttttt
      241 ctcaagttcc tgttggagct ccaggaggaa ggctggttcc gtggcttttt ggatgcccta
      301 gaccatgcag gttattctgg actttatgaa gccattgaaa gttgggattt caaaaaaatt
      361 gaaaagttgg aggagtatag attactttta aaacgtttac aaccagaatt taaaaccaga
      421 attatcccaa ccgatatcat ttctgatctg tctgaatgtt taattaatca ggaatgtgaa
      481 gaaattctac agatttgctc tactaagggg atgatggcag gtgcagagaa attggtggaa
      541 tgccttctca gatcagacaa ggaaaactgg cccaaaactt tgaaacttgc tttggagaaa
      601 gaaaggaaca agttcagtga actgtggatt gtagagaaag gtataaaaga tgttgaaaca
      661 gaagatcttg aggataagat
//
