LOCUS       sequence-83839-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human Phosphomevalonate kinase (PMK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Herdendorf TJ, Miziorko HM
  TITLE     Phosphomevalonate kinase: functional investigation of the 
            recombinant human enzyme.
  JOURNAL   Biochemistry. 2006 Mar 14;45(10):3235-42.
  PUBMED    16519518
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-03-14"; volume: "45(10)"; pages: 
            "3235-42"
COMMENT     Sequence Label: Human Phosphomevalonate kinase (PMK)
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    68..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             107..129
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             148..165
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             175..192
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
       61 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
      121 agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag cggcctggtg
      181 ccgcgcggca gccatatggc cccgctggga ggcgccccgc ggctggtact gctgttcagc
      241 ggcaagagga aatccgggaa ggacttcgtg accgaggcgc tgcagagcag acttggagct
      301 gatgtctgtg ctgtcctccg gctctctggt ccactcaagg aacagtatgc tcaggagcat
      361 ggcttgaact tccagagact cctggacacc agcacctaca aggaggcctt tcggaaggac
      421 atgatccgct ggggagagga gaaacgccag gctgacccag gcttcttttg caggaagatt
      481 gtggagggca tctcccagcc catctggctg gtgagtgaca cacggagagt gtctgacatc
      541 cagtggtttc gggaggccta tggggccgtg acgcagacgg tccgcgttgt agcgttggag
      601 cagagccgac agcagcgggg ctgggtgttc acgccagggg tggacgatgc tgagtcagaa
      661 tgtggcctgg acaacttcgg ggactttgac tgggtcatcg agaaccatgg agttgaacag
      721 cgcctggagg agcagttgga gaacctgata gaatttatcc gctccagact ttaggatccg
//
