LOCUS       sequence-83861-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) 
            mCherry::Asp terminator + ColE1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1277
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..73
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(94..112)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        180..284
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(503..522)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catttttttt cctccttagt cgacagtatc tctatcactg catagggatg tcaatctcta
       61 tcactgatag ggactcgagg tgaagacgaa agggcctcgt gatacgccta tttttatagg
      121 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      181 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      241 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      301 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      361 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      421 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      481 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
      541 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
      601 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
      661 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
      721 taaccgctt
//
