LOCUS       sequence-83994-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1756
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..647)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     555..574
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      complement(788..815)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtactaccct gtacgattaa agcttaagac ccactttcac atttaagttg tttttctaat
       61 ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg gtgatcaaat
      121 aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt ttccctttct
      181 tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca
      241 gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa aaaggctaat
      301 tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg tacttttgct
      361 ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg taaaaaatct
      421 tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa catctcaatg
      481 gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt aggctgctct
      541 acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac ctcattaagc
      601 agctctaatg cgctgttaat cactttactt ttatctaatc tagacatttt cctccttacc
      661 cgggtgtgtg gaatccatta taaccgccaa gcataaaaat gtcaacaacg acggtgctcg
      721 aggatcctga gcggatacat atttgaatgt atttagaaaa ataaacaaaa gagtttgtag
      781 aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc tggcagttta
      841 tggcgggcgt cctgcccg
//
