LOCUS       sequence-84028-e         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of human LIN-41 / TRIM71 containing cysteine 
            to alanine point mutations of all 7 cysteines in the RING domain and
            3 N-terminal HA tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, 
            Narita M, Srivastava D, Yamanaka S
  TITLE     The let-7/LIN-41 pathway regulates reprogramming to human induced 
            pluripotent stem cells by controlling expression of 
            prodifferentiation genes.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 
            10.1016/j.stem.2013.11.001. Epub 2013 Nov 14.
  PUBMED    24239284
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11.001"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "40-52"
COMMENT     Sequence Label: pMXs-hs-3xHA-7CtoA-LIN-41
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..200
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gtgaaggctg ccgaccccgg gggtggacca tcctctagac tgccggatct agctagttaa
       61 ttaaggatcc cagtgtggtg gtacgggaat tcctgcaggc caccatggca tacccatacg
      121 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat
      181 atgacgttcc agattacgct atggcttcgt tccccgagac cgatttccag atcgccttgc
      241 tggccaagga gatgtgcggc tcgccggcgc cgctctcctc caactcgtcc gcgtcgtcgt
      301 cctcctcgca gacgtccacg tcgtcggggg gcggcggcgg gggccctggg gcggcggcgc
      361 gccgcctaca cgtcctgccc gccctgcacg ccttcgcccg ccccgccctc gaggcgcacc
      421 ggctgccggc ggcgggcggc ggcgcggcgg gagagccgct caagctgcgc gcccccgtgg
      481 ccgaccagaa agtagtgcta gccgaggcgg cgggtatgga cgcgctgcct tcgtccgcct
      541 tcctgcttag caacctgctc gacgcggtgg tggccactgc cgacgagccg ccgcccaaga
      601 acgggcgcgc cggcgctccg gcgggagcgg gcgg
//
