LOCUS       sequence-84057-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] 
            (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is 
            cloned in between PmeI/SalI.Confers ampicillin resistance to 
            DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pLpC-Reg-L-IFP-F[2]+Venus (mammalian)
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..766)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 taataaatct tttattttat cggccctcta gatgcatgct cgagtcgact tatttgtaca
       61 attcatccat accatgggta ataccagcag cagtaacaaa ttctaacaag accatgtggt
      121 ctctcttttc gtttggatct ttggataatt tagattgata ggataagtaa tggttgtctg
      181 gtaacaagac tggaccatca ccaattggag tattttgttg ataatggtca gctaattgaa
      241 caccaccatc ttcaatgttg tgtctaattt tgaagttagc tttgatacca ttcttttgtt
      301 tgtcagcagt gatgtaaaca ttgtgagagt tatagttgta ttccaatttg tgacctaaaa
      361 tgttaccatc ttctttaaaa tcaatacctt ttaattcgat tctattaact aaggtatcac
      421 cttcaaactt gacttcagct ctggtcttgt agttaccgtc atctttgaaa aaaatagttc
      481 tttcttgaac ataaccttct ggcatggcag acttgaaaaa gtcatgttgt ttcatatgat
      541 ctgggtatct agcaaaacat tgcaaaccat aacctaaagt agtgactaag gttggccatg
      601 gaactggcaa tttaccagta gtacaaatca attttaaggt caatttaccg taagtagcat
      661 caccttcacc ttcaccggag acagaaaatt tgtgaccatt aacatcacca tctaattcaa
      721 ccaaaattgg gacaacacca gtgaataatt cttcaccttt agacatgttt aaacccggga
      781 tatcagatct atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg gggggcctag
      841 acgttttttt aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga
      901 tcccatacaa tggggtacct tctgggcatc cttcagcccc ttggttgaat acgccttgag
      961 gagagccatt tgactctttc cac
//
