LOCUS       sequence-84149-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA silencing plasmid with eGFP transfection marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Schnizler MK, Schnizler K, Zha XM, Hall DD, Wemmie JA, Hell JW, 
            Welsh MJ
  TITLE     The cytoskeletal protein alpha-actinin regulates acid-sensing ion 
            channel 1a through a C-terminal interaction.
  JOURNAL   J Biol Chem. 2009 Jan 30;284(5):2697-705. doi: 
            10.1074/jbc.M805110200. Epub 2008 Nov 21.
  PUBMED    19028690
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M805110200"; journalName: "J Biol Chem"; date: 
            "2009-01-30- 30"; volume: "284"; issue: "5"; pages: "2697-705"
COMMENT     Sequence Label: pSIL-eGFP
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..71
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(105..121)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(106..122)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(134..447)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(195..215)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(444..460)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(517..538)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cattaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgctctaga
       61 actagtggat cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc
      121 gagggggggc ccaaacaagg cttttctcca agggatattt atagtctcaa aacacacaat
      181 tactttacag ttagggtgag tttccttttg tgctgttttt taaaataata atttagtatt
      241 tgtatctctt atagaaatcc aagcctatca tgtaaaatgt agctagtatt aaaaagaaca
      301 gattatctgt cttttatcgc acattaagcc tctatagtta ctaggaaata ttatatgcaa
      361 attaaccggg gcaggggagt agccgagctt ctcccacaag tctgtgcgag ggggccggcg
      421 cgggcctaga gatggcggcg tcggatccac tagttctaga gcgggtacct agtacttgta
      481 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      541 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
      601 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      661 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
      721 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
      781 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      841 gccctcgaac ttcacctcgg cgcggg
//
