LOCUS       sequence-84174-e        2330 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2330)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ALK1-N-13
FEATURES             Location/Qualifiers
     source          1..2330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1578..1587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1584..1586,1587..1589,1590..2303)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1629..1650)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1890..1909)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2237..2258
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcagatctc gagctcaagc ttcgaattca ccgccatgac cttgggctcc cccaggaaag
       61 gccttctgat gctgctgatg gccttggtga cccagggaga ccctgtgaag ccgtctcggg
      121 gcccgctggt gacctgcacg tgtgagagcc cacattgcaa ggggcctacc tgccgggggg
      181 cctggtgcac agtagtgctg gtgcgggagg aggggaggca cccccaggaa catcggggct
      241 gcgggaactt gcacagggag ctctgcaggg ggcgccccac cgagttcgtc aaccactact
      301 gctgcgacag ccacctctgc aaccacaacg tgtccctggt gctggaggcc acccaacctc
      361 cttcggagca gccgggaaca gatggccagc tggccctgat cctgggcccc gtgctggcct
      421 tgctggccct ggtggccctg ggtgtcctgg gcctgtggca tgtccgacgg aggcaggaga
      481 agcagcgtgg cctgcacagc gagctgggag agtccagtct catcctgaaa gcatctgagc
      541 agggcgacag catgttgggg gacctcctgg acagtgactg caccacaggg agtggctcag
      601 ggctcccctt cctggtgcag aggacagtgg cacggcaggt tgccttggtg gagtgtgtgg
      661 gaaaaggccg ctatggcgaa gtgtggcggg gcttgtggca cggtgagagt gtggccgtca
      721 agatcttctc ctcgagggat gaacagtcct ggttccggga gactgagatc tataacacag
      781 tgttgctcag acacgacaac atcctaggct tcatcgcctc agacatgacc tcccgcaact
      841 cgagcacgca gctgtggctc atcacgcact accacgagca cggctccctc tacgactttc
      901 tgcagagaca gacgctggag ccccatctgg ctctgaggct agctgtgtcc gcggcatgcg
      961 gcctggcgca cctgcacgtg gagatcttcg gtacacaggg caaaccagcc attgcccacc
     1021 gcgacttcaa gagccgcaat gtgctggtca agagcaacct gcagtgttgc atcgccgacc
     1081 tgggcctggc tgtgatgcac tcacagggca gcgattacct ggacatcggc aacaacccga
     1141 gagtgggcac caagcggtac atggcacccg aggtgctgga cgagcagatc cgcacggact
     1201 gctttgagtc ctacaagtgg actgacatct gggcctttgg cctggtgctg tgggagattg
     1261 cccgccggac catcgtgaat ggcatcgtgg aggactatag accacccttc tatgatgtgg
     1321 tgcccaatga ccccagcttt gaggacatga agaaggtggt gtgtgtggat cagcagaccc
     1381 ccaccatccc taaccggctg gctgcagacc cggtcctctc aggcctagct cagatgatgc
     1441 gggagtgctg gtacccaaac ccctctgccc gactcaccgc gctgcggatc aagaagacac
     1501 tacaaaaaat tagcaacagt ccagagaagc ctaaagtgat tcaaggcagc gcaggcggaa
     1561 gcggggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     1621 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1681 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1741 agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg cagtgcttcg
     1801 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1861 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1921 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1981 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aaggtctata
     2041 tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc cacaacatcg
     2101 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2161 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
     2221 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2281 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
//
