LOCUS       sequence-84175-e        3605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3605)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Alpha1-Catenin-C-18
FEATURES             Location/Qualifiers
     source          1..3605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      481 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
      541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctggc tccagcgcag
      781 gcagcgcatc cggcggaagc ggaagcactg ccgtccacgc aggcaacata aacttcaaat
      841 gggaccccaa aagtctggag attaggactc tggctgttga aagactgttg gagcctcttg
      901 ttacacaggt tacaaccctg gtaaacacca atagtaaagg gccgtctaat aaaaagagag
      961 gccgttctaa gaaggcccat gttttggctg catctgttga acaagcaact gagaatttct
     1021 tggaaaaggg ggataaaatt gcaaaagaga gccagtttct caaggaggag cttgtggttg
     1081 ctgtagaaga tgttcgaaaa caaggagatt tgatgaagag cgctgctggg gagtttgcag
     1141 atgatccatg ctcttctgtg aagcgaggca acatggtccg ggcagctcga gctttgctct
     1201 ctgccgtcac ccggctgctg attctggctg acatggcaga tgtctacaaa ttacttgttc
     1261 agctgaaagt tgtggaagat ggtatattga aactgaggaa tgctggcaat gaacaagact
     1321 tagggataca gtacaaagct ctgaaaccag aagtagataa gctgaacatc atggcagcaa
     1381 aaagacaaca ggaactaaaa gatgtgggca atcgtgatca gatggctgca gctagaggaa
     1441 tcctgcagaa aaatgttcca atcctctata ctgcatccca ggcatgtcta caacaccctg
     1501 atgttgcagc ctataaggcc aaccgggact tgatatacaa gcagctgcag caggccgtca
     1561 caggaatttc taatgcagcc caggctactg catcagatga tgctgcccag caccagggtg
     1621 gcagtggcgg agagctggca tacgctctca acaactttga taaacaaatc attgtggacc
     1681 ccttgagctt cagcgaggag cgctttaggc cgtccctgga ggagcgcctg gaaagcatta
     1741 ttagtggggc tgccctgatg gctgactcat cctgcacacg tgatgaccgt cgggagcgca
     1801 ttgtggcaga gtgtaatgct gtccgccagg ctctacagga cctgctctca gaatacatgg
     1861 gcaacgctgg acgtaaagaa agaagtgatg cactcaactc tgcaatagat aaaatgacca
     1921 agaagaccag ggacttgcgt agacagcttc gcaaagctgt catggaccac gtatcagatt
     1981 ctttcctgga gaccaatgtc cctctattag tactgattga agctgcaaag aatggaaatg
     2041 agaaagaagt taaggaatat gcccaagttt ttcgtgaaca tgccaacaaa ctgattgagg
     2101 ttgccaacct ggcctgttca atctccaaca atgaagaagg cgtgaagctt gtccgaatgt
     2161 ctgcaagcca gttagaagcg ctgtgtcctc aggttatcaa tgctgcattg gcgttggcag
     2221 caaagccgca gagtaaactg gcccaagaga acatggatct ttttaaagag caatgggaaa
     2281 agcaagtccg tgttctcaca gatgctgttg atgacattac ttccatcgat gacttcttgg
     2341 ctgtctcaga gaaccacatt ttagaagatg tgaacaagtg tgttattgct ctccaagaga
     2401 aagacgtgga tgggctggat cgcactgccg gtgccatccg aggccgggca gcccgagtca
     2461 ttcatgtagt cacctcagag atggacaact acgagccagg agtttacaca gagaaggttc
     2521 tggaagccac caagctcctc tccaacacag tcatgccgcg cttcactgag caggtggaag
     2581 cagctgtgga agccctcagc tcggacccgg cccagcccat ggatgagaat gagttcatcg
     2641 acgcctcccg tctggtgtac gacggcatcc gggacatccg gaaagcagtg ctgatgatca
     2701 ggacccccga ggagttggac gactctgact tcgagactga agactttgat gtcagaagca
     2761 ggaccagtgt ccagacagaa gatgaccagc tgatagctgg ccagagcgcc cgggcgatca
     2821 tggctcagct tcctcaggag caaaaagcaa agattgcaga acaggtggcc agtttccagg
     2881 aagagaagag caagctggat gctgaagtgt ccaagtggga tgacagtggc aatgacatca
     2941 ttgtgctggc caagcagatg tgcatgatca tgatggagat gaccgacttc acccgaggca
     3001 aaggtccact caaaaacacc tctgacgtga tcagtgctgc caagaaaatt gcggaggcgg
     3061 gatccaggat ggacaagctc ggccggacca tcgcagacca ttgcccagac tcggcatgca
     3121 agcaggacct gctggcctac cttcagcgca tcgctctcta ctgccaccag ctcaacatct
     3181 gcagcaaagt caaggctgaa gtacagaacc ttggtggaga gctggtcgtc tctggggtgg
     3241 acagcgccat gtccctgatc caggcggcca agaacttgat gaatgctgtt gtgcagacag
     3301 tgaaggcgtc ctacgtggct tccaccaaat accagaagtc acaggggatg gcttccttga
     3361 accttcctgc tgtgtcatgg aagatgaagg cccctgagaa gaagccgttg gtgaagagag
     3421 agaagcagga tgagacgcag accaagatta aacgagcttc tcagaagaaa cacgtgaacc
     3481 cagtgcaggc cctgagcgag ttcaaagcca tggacagcat ctgagaattc tgcagtcgac
     3541 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
     3601 ttgta
//
