LOCUS       sequence-84194-e        1654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1654)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ATG5-N-14
FEATURES             Location/Qualifiers
     source          1..1654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      904..913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(910..912,913..915,916..1629)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(955..976)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1216..1235)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1563..1584
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat ctcgagaccg ccatgacaga tgacaaagat
       61 gtgcttcgag atgtgtggtt tggacgaatt ccaacttgtt tcacgctata tcaggatgag
      121 ataactgaaa gggaagcaga accatactat ttgcttttgc caagagtaag ttatttgacg
      181 ttggtgactg acaaagtgaa aaagcacttt cagaaggtta tgagacaaga agacattagt
      241 gagatatggt ttgaatatga aggcacacca ctgaaatggc attatccaat tggtttgcta
      301 tttgatcttc ttgcatcaag ttcagctctt ccttggaaca tcacagtaca ttttaagagt
      361 tttccagaaa aagaccttct gcactgtcca tctaaggatg caattgaagc tcattttatg
      421 tcatgtatga aagaagctga tgctttaaaa cataaaagtc aagtaatcaa tgaaatgcag
      481 aaaaaagatc acaagcaact ctggatggga ttgcaaaatg acagatttga ccagttttgg
      541 gccatcaatc ggaaactcat ggaatatcct gcagaagaaa atggatttcg ttatatcccc
      601 tttagaatat atcagacaac gactgaaaga cctttcattc agaagctgtt tcgtcctgtg
      661 gctgcagatg gacagttgca cacactagga gatctcctca aagaagtttg tccttctgct
      721 attgatcctg aagatgggga aaaaaagaat caagtgatga ttcatggaat tgagccaatg
      781 ttggaaacac ctctgcagtg gctgagtgaa catctgagct acccggataa ttttcttcat
      841 attagtatca tcccacagcc aacagatggg agtggcagtc gcggacagag tggtggaccg
      901 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      961 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1021 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1081 tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac
     1141 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1201 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1261 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1321 ctggggcaca agctggagta caactacaac agccacaagg tctatatcac cgccgacaag
     1381 cagaagaacg gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg
     1441 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1501 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     1561 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1621 tacaagtaag cggccgcgac tctagatcat aatc
//
