LOCUS       sequence-84245-e        3976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3976)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Desmoglein1-N-18
FEATURES             Location/Qualifiers
     source          1..3976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3225..3234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3231..3233,3234..3236,3237..3950)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3276..3297)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3537..3556)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3884..3905
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggaccgtcag atccgctagc gtcgccacca tggactggag tttcttcaga gtagttgcag
       61 tgctgttcat ttttctggtg gtggtagaag ttaacagtga gttccgaatc caggtaagag
      121 attataacac taaaaatggc accatcaaat ggcattcaat ccgaaggcag aaacgtgaat
      181 ggatcaagtt cgcagcagcc tgtcgtgaag gtgaagacaa ctcaaagagg aacccaatcg
      241 ccaaaattca ctcagattgt gctgcaaacc agcaagttac ataccgcatc tctggagtag
      301 gaattgatca gccaccatat gggatctttg tcattaatca gaaaactggt gaaattaata
      361 taacatccat agttgatcga gaggtcactc ctttcttcat tatctactgc cgagctctga
      421 actcaatggg ccaagattta gagaggcctc tagagctcag agtcagggtt ttggatataa
      481 atgacaaccc tccagtgttt tcaatggcta catttgcagg acaaatagaa gaaaattcta
      541 atgcaaatac actggtgatg atactcaatg ctactgacgc agatgaaccg aacaatttga
      601 actcaaaaat agccttcaag attataagac aagaaccttc agattcacca atgtttatta
      661 tcaacagaaa tactggagaa attcgaacga tgaataattt tctagacaga gagcaatacg
      721 gccagtatgc tcttgctgta agaggctctg accgagatgg tggggcagat ggcatgtcag
      781 cggaatgtga gtgcaacatt aaaatcctcg atgtcaatga taatatccct tacatggaac
      841 agtcttcata taccatagaa attcaagaaa atactctaaa ttcaaatttg ctcgaaatta
      901 gagtaattga tttggatgaa gagttctcag ctaactggat ggcagtaatt ttctttatct
      961 ctggaaatga aggaaattgg tttgagatag aaatgaatga aagaacaaat gtgggaattt
     1021 taaaggttgt taagccctta gattatgaag ctatgcagag tctgcaactc agtattggtg
     1081 tcagaaataa agctgaattt catcattcaa ttatgtctca atataaactg aaagcatctg
     1141 caatttctgt gactgtgtta aatgtaattg aaggcccagt gtttcgtcca ggttcaaaga
     1201 catatgttgt aactggtaat atgggatcaa atgataaagt gggagacttt gtagctactg
     1261 acctggacac aggtagacct tcaacgactg ttaggtatgt aatgggaaat aatccagctg
     1321 acctgcttgc tgttgattca agaacaggca aactcacttt gaaaaataaa gttaccaagg
     1381 aacagtacaa tatgctcgga ggaaaatacc aaggaacgat tctctctata gatgataatc
     1441 ttcaaagaac ttgcactggt acaattaata ttaacattca aagttttggt aatgacgaca
     1501 ggactaatac agagccgaac actaaaatta ctaccaatac tggcagacaa gaaagtactt
     1561 cttccactaa ctatgatacc agcacaactt ctactgactc tagccaagta tattcttctg
     1621 aacccggaaa cggagccaaa gatttgttat cagacaatgt acattttggt cctgctggca
     1681 ttggactcct catcatggga ttcttggtct taggattggt cccatttttg atgatctgtt
     1741 gtgattgtgg aggtgctcct cgtagtgcag ctggctttga gcctgttccc gaatgttcag
     1801 atggagcaat tcattcatgg gcagtagaag gaccacagcc tgaacccagg gatataacca
     1861 ctgtcatacc acaaatacca cctgataacg caaatataat tgaatgcatt gacaactcag
     1921 gagtttatac aaatgagtat ggtggcagag aaatgcaaga cctgggagga ggagagagaa
     1981 tgacaggatt tgaactaaca gagggagtta aaacttcagg aatgcctgag atatgtcaag
     2041 aatactctgg aacattaaga agaaattcta tgagggaatg tagagaagga ggtctgaata
     2101 tgaatttcat ggaaagctac ttctgtcaga aagcatatgc ttacgcagat gaagatgaag
     2161 gacgcccatc taatgactgt ttgctcatat atgacatcga aggtgtaggt tcccctgctg
     2221 gctctgtggg ttgttgtagc ttcattggag aagacctgga tgacagcttc ttggataccc
     2281 tgggacctaa atttaagaag ttggcagaca tcagcctagg aaaagaatca tatccagacc
     2341 ttgatccttc ttggccacca caaagcactg aaccagtttg ccttcctcag gaaacagagc
     2401 ccgttgttag tggacaccca ccaatctccc cacatttcgg cactaccaca gtaatttctg
     2461 agagcaccta tccctcggga cctggtgtac tgcatcctaa gcctattctc gatcctctgg
     2521 gctatggtaa tgtcactgtg accgagtctt acaccacctc tgacactctg aagccctctg
     2581 tgcacgttca cgataaccga ccagcatcaa acgtggtagt gacagagaga gtggtcggcc
     2641 caatctctgg cgctgatttg catggaatgt tagagatgcc tgacttgcga gatgggtcga
     2701 atgttatagt gacagaaagg gtaatagcac caagctctag tctacccacc tctctgacta
     2761 tccatcatcc tagagagtct tcaaatgtgg tagtgacaga aagagtaatc caaccaactt
     2821 ccggcatgat aggtagtctg agtatgcacc ccgagttagc caatgcccac aatgtcattg
     2881 tgacagagag ggttgtttct ggtgctggcg taactggaat tagtggcacc actgggatca
     2941 gcggtggcat aggcagcagt ggcctggttg gcaccagcat gggtgctggg agcggtgccc
     3001 tgagtggagc tggcataagt ggtggtggca ttggcctgag cagcttggga gggacagcca
     3061 gcattggcca catgaggagt tcctctgacc atcactttaa ccaaaccatt gggtccgcct
     3121 cccctagcac agctcgaagt cgaatcacaa agtatagtac cgtgcaatat agcaagagca
     3181 gcggtggagc aagcgcagcc agtggtagcg cggatccacc ggtcgccacc atggtgagca
     3241 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     3301 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     3361 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     3421 ccttgaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     3481 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3541 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3601 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3661 acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     3721 tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3781 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     3841 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3901 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gcggccgcga
     3961 ctctagatca taatca
//
