LOCUS       sequence-84264-e        2582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions/Membrane, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2582)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Ezrin-N-14
FEATURES             Location/Qualifiers
     source          1..2582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1818..1827
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1824..1826,1827..1829,1830..2543)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1869..1890)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2130..2149)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2477..2498
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgccga aaccaatcaa tgtccgagtt accaccatgg
       61 atgcagagct ggagtttgca atccagccaa atacaactgg aaaacagctt tttgatcagg
      121 tggtaaagac tatcggcctc cgggaagtgt ggtactttgg cctccactat gtggataata
      181 aaggatttcc tacctggctg aagctggata agaaggtgtc tgcccaggag gtcaggaagg
      241 agaatcccct ccagttcaag ttccgggcca agttctaccc tgaagatgtg gctgaggagc
      301 tcatccagga catcacccag aaacttttct tcctccaagt gaaggaagga atccttagcg
      361 atgagatcta ctgcccccct gagactgccg tgctcttggg gtcctacgct gtgcaggcca
      421 agtttgggga ctacaacaaa gaagtgcaca agtctgggta cctcagctct gagcggctga
      481 tccctcaaag agtgatggac cagcacaaac ttaccaggga ccagtgggag gaccggatcc
      541 aggtgtggca tgcggaacac cgtgggatgc tcaaagataa tgctatgttg gaatacctga
      601 agattgctca ggacctggaa atgtatggaa tcaactattt cgagataaaa aacaagaaag
      661 gaacagacct ttggcttgga gttgatgccc ttggactgaa tatttatgag aaagatgata
      721 agttaacccc aaagattggc tttccttgga gtgaaatcag gaacatctct ttcaatgaca
      781 aaaagtttgt cattaaaccc atcgacaaga aggcacctga ctttgtgttt tatgccccac
      841 gtctgagaat caacaagcgg atcctgcagc tctgcatggg caaccatgag ttgtatatgc
      901 gccgcaggaa gcctgacacc atcgaggtgc agcagatgaa ggcccaggcc cgggaggaga
      961 agcatcagaa gcagctggag cggcaacagc tggaaacaga gaagaaaagg agagaaaccg
     1021 tggagagaga gaaagagcag atgatgcgcg agaaggagga gttgatgctg cggctgcagg
     1081 actatgagga gaagacaaag aaggcagaga gagagctctc ggagcagatt cagagggccc
     1141 tgcagctgga ggaggagagg aagcgggcac aggaggaggc cgagcgccta gaggctgacc
     1201 gtatggctgc actgcgggct aaggaggagc tggagagaca ggcggtggat cagataaaga
     1261 gccaggagca gctggctgcg gagcttgcag aatacactgc caagattgcc ctcctggaag
     1321 aggcgcggag gcgcaaggag gatgaagttg aagagtggca gcacagggcc aaagaagccc
     1381 aggatgacct ggtgaagacc aaggaggagc tgcacctggt gatgacagca cccccgcccc
     1441 caccaccccc cgtgtacgag ccggtgagct accatgtcca ggagagcttg caggatgagg
     1501 gcgcagagcc cacgggctac agcgcggagc tgtctagtga gggcatccgg gatgaccgca
     1561 atgaggagaa gcgcatcact gaggcagaga agaacgagcg tgtgcagcgg cagctgctga
     1621 cgctgagcag cgagctgtcc caggcccgag atgagaataa gaggacccac aatgacatca
     1681 tccacaacga gaacatgagg caaggccggg acaagtacaa gacgctgcgg cagatccggc
     1741 agggcaacac caagcagcgc atcgacgagt tcgaggccct ggcatccggc ggtagcggcg
     1801 gtggcagcgg accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     1861 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1921 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1981 agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg cagtgcttcg
     2041 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2101 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2161 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2221 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aaggtctata
     2281 tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc cacaacatcg
     2341 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2401 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
     2461 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2521 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca
     2581 tt
//
