LOCUS       sequence-84274-e        2445 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Associated Kinase, Excitation: 487, Emission:
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2445)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-FYN2-N-10
FEATURES             Location/Qualifiers
     source          1..2445
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1670..1679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1676..1678,1679..1681,1682..2395)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1721..1742)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1982..2001)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2329..2350
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cccatgcagt gacgtcagat ccgctagcgc caccatgggc tgtgtgcaat gtaaggataa
       61 agaagcaaca aaactgacgg aggagaggga cggcagcctg aaccagagct ctgggtaccg
      121 ctatggcaca gaccccaccc ctcagcacta ccccagcttc ggtgtgacct ccatccccaa
      181 ctacaacaac ttccacgcag ccgggggcca aggactcacc gtctttggag gtgtgaactc
      241 ttcgtctcat acggggacct tgcgtacgag aggaggaaca ggagtgacac tctttgtggc
      301 cctttatgac tatgaagcac ggacagaaga tgacctgagt tttcacaaag gagaaaaatt
      361 tcaaatattg aacagctcgg aaggagattg gtgggaagcc cgctccttga caactggaga
      421 gacaggttac attcccagca attatgtggc tccagttgac tctatccagg cagaagagtg
      481 gtactttgga aaacttggcc gaaaagatgc tgagcgacag ctattgtcct ttggaaaccc
      541 aagaggtacc tttcttatcc gcgagagtga aaccaccaaa ggtgcctatt cactttctat
      601 ccgtgattgg gatgatatga aaggagacca tgtcaaacat tataaaattc gcaaacttga
      661 caatggtgga tactacatta ccacccgggc ccagtttgaa acacttcagc agcttgtaca
      721 acattactca gagagagctg caggtctctg ctgccgccta gtagttccct gtcacaaagg
      781 gatgccaagg cttaccgatc tgtctgtcaa aaccaaagat gtctgggaaa tccctcgaga
      841 atccctgcag ttgatcaaga gactgggaaa tgggcagttt ggggaagtat ggatgggtac
      901 ctggaatgga aacacaaaag tagccataaa gactcttaaa ccaggcacaa tgtcccccga
      961 atcattcctt gaggaagcgc agatcatgaa gaagctgaag cacgacaagc tggtccagct
     1021 ctatgcagtg gtgtctgagg agcccatcta catcgtcacc gagtatatga acaaaggaag
     1081 tttactggat ttcttaaaag atggagaagg aagagctctg aaattaccaa atcttgtgga
     1141 catggcagca caggtggctg caggaatggc ttacatcgag cgcatgaatt atatccatag
     1201 agatctgcga tcagcaaaca ttctagtggg gaatggactc atatgcaaga ttgctgactt
     1261 cggattggcc cgattgatag aagacaatga gtacacagca agacaaggtg caaagttccc
     1321 catcaagtgg acggcccccg aggcagccct gtacgggagg ttcacaatca agtctgacgt
     1381 gtggtctttt ggaatcttac tcacagagct ggtcaccaaa ggaagagtgc catacccagg
     1441 catgaacaac cgggaggtgc tggagcaggt ggagcgaggc tacaggatgc cctgcccgca
     1501 ggactgcccc atctctctgc atgagctcat gatccactgc tggaaaaagg accctgaaga
     1561 acgccccact tttgagtact tgcagagctt cctggaagac tactttaccg cgacagagcc
     1621 ccagtaccaa cctggtgaaa acctggcatc cagcggggat ccaccggtcg ccaccatggt
     1681 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1741 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1801 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1861 gaccaccttg acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     1921 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1981 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2041 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     2101 ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat
     2161 caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     2221 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2281 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2341 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagcggc
     2401 cgcgactcta gatcataatc agccatacca cattgagagt tgcac
//
