LOCUS       sequence-84275-e        2035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin , Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2035)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Gamma-Actin2-C-18
FEATURES             Location/Qualifiers
     source          1..2035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(46..48,49..51,52..762)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(91..112)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(352..371)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     699..720
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1945..1997
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tcatggctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      121 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      181 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg
      241 acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      301 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      361 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      481 tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac
      541 ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      661 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      721 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggagg gtccagcggc
      781 ggtggatctg ggtccagcgg aagtagcggc agcggctgtg aagaagagac caccgccctt
      841 gtgtgtgaca atggctctgg cctgtgcaag gcaggctttg caggagatga tgcccccagg
      901 gctgtcttcc cctccattgt gggccgccct agacatcagg gtgtgatggt gggaatgggc
      961 cagaaagaca gctatgtggg ggacgaggct cagagcaagc gtgggatcct aactctcaag
     1021 taccctattg aacatggcat catcaccaac tgggatgaca tggagaagat ctggcaccac
     1081 tccttctaca atgagcttcg agtagcacca gaagagcacc ccaccctgct cacagaggcc
     1141 cccctaaacc ccaaagcaaa cagggagaag atgacccaga tcatgttcga aaccttcaat
     1201 gtccctgcca tgtatgttgc tattcaggct gtgctctcac tctatgcatc cggccgtacc
     1261 acaggcatcg tcctggattc gggggatggc gtcacccaca atgtccccat ctatgagggc
     1321 tatgcactgc cccatgccat catgcgtctt gacctggctg gacgggatct cacagactac
     1381 ctcatgaaga ttctcacaga aagaggctat tcctttgtga ccacagctga gagagaaatt
     1441 gtacgagaca tcaaggagaa gctgtgctat gtagccctgg atttcgagaa tgagatggcc
     1501 acagcagctt catcttcttc cctggagaaa agctacgagt tgcctgatgg gcaggtcatc
     1561 actattggca acgaacgctt ccgctgcccg gagaccctct tccagccttc cttcattggc
     1621 atggagtcag ctggaattca tgaaacaaca tacaattcca tcatgaagtg tgacattgac
     1681 atccgcaaag atttgtatgc taacaatgtc ctctctgggg gcactaccat gtaccctggc
     1741 attgctgaca ggatgcagaa ggaaatcaca gccttggctc ccagcaccat gaagatcaag
     1801 attatcgctc ctcctgagcg gaagtactca gtctggattg gcggctccat cctggcctct
     1861 ctctccacct tccagcaaat gtggatcagc aagccagagt atgatgaggc agggccctcc
     1921 attgtccaca ggaaatgctt ctaactcgag ctcaagcttc gaattctgca gtcgacggta
     1981 ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat accac
//
