LOCUS       sequence-84277-e        2144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2144)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-GFAP-C-18
FEATURES             Location/Qualifiers
     source          1..2144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(46..48,49..51,52..762)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(91..112)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(352..371)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     699..720
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccatgcttag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      121 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      181 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg
      241 acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      301 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      361 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      481 tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac
      541 ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      661 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      721 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact cagatctggc
      781 tccagcgcag gcagcgcatc cggcggaagc ggaagcgaga ggagacgcat cacctccgct
      841 gctcgccgct cctacgtctc ctcaggggag atgatggtgg ggggcctggc tcctggccgc
      901 cgtctgggtc ctggcacccg cctctccctg gctcgaatgc cccctccact cccaacccgg
      961 gtggatttct ccctggctgg ggcactcaat gctggcttca aggagacccg ggccagtgag
     1021 cgggcagaga tgatggagct caatgaccgc tttgccagct acatcgagaa ggttcgcttc
     1081 ctggaacagc aaaacaaggc gctggctgct gagctgaacc agctgcgggc caaggagccc
     1141 accaagctgg cagacgtcta ccaggctgag ctgcgagagc tgcggctgcg gctcgatcaa
     1201 ctcaccgcca acagcgcccg gctggaggtt gagagggaca atctggcaca ggacctggcc
     1261 actgtgaggc agaagctcca ggatgaaacc aacctgaggc tggaagccga gaacaacctg
     1321 gctgcctata gacaggaagc agatgaagcc accctggccc gtctggatct ggagaggaag
     1381 attgagtcgc tggaggagga gatccggttc ttgaggaaga tccacgagga ggaggttcgg
     1441 gaactccagg agcagctggc ccgacagcag gtccatgtgg agcttgacgt ggccaagcca
     1501 gacctcaccg cagccctgaa agagatccgc acgcagtatg aggcaatggc gtccagcaac
     1561 atgcatgaag ccgaagagtg gtaccgctcc aagtttgcag acctgacaga cgctgctgcc
     1621 cgcaacgcgg agctgctccg ccaggccaag cacgaagcca acgactaccg gcgccagttg
     1681 cagtccttga cctgcgacct ggagtctctg cgcggcacga acgagtccct ggagaggcag
     1741 atgcgcgagc aggaggagcg gcacgtgcgg gaggcggcca gttatcagga ggcgctggcg
     1801 cggctggagg aagaggggca gagcctcaag gacgagatgg cccgccactt gcaggagtac
     1861 caggacctgc tcaatgtcaa gctggccctg gacatcgaga tcgccaccta caggaagctg
     1921 ctagagggcg aggagaaccg gatcaccatt cccgtgcaga ccttctccaa cctgcagatt
     1981 cgagaaacca gcctggacac caagtctgtg tcagaaggcc acctcaagag gaacatcgtg
     2041 gtgaagaccg tggagatgcg ggatggagag gtcattaagg agtccaagca ggagcacaag
     2101 gatgtgatgt gaggatccac cggatctaga taactgatca taat
//
