LOCUS       sequence-84290-e        1959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Adaptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1959)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Homer1-N-18
FEATURES             Location/Qualifiers
     source          1..1959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1183..1192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1189..1191,1192..1194,1195..1908)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1234..1255)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1495..1514)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1842..1863
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acaattgcta gtgacgtcag atccgctagc gccaccatgg gggaacaacc tatcttcagc
       61 actcgagctc atgtcttcca gatcgaccca aacacaaaga agaactgggt acccaccagc
      121 aagcatgcag ttactgtgtc ttatttctat gacagcacaa ggaatgtgta taggataatc
      181 agtttagacg gctcaaaggc aataataaat agcaccatca ctccaaacat gacatttact
      241 aaaacatctc aaaagtttgg ccaatgggct gatagccggg caaacactgt ttatggactg
      301 ggattctcct ctgagcatca tctctcaaaa tttgcagaaa agtttcagga atttaaagaa
      361 gctgctcggc tggcaaagga gaagtcgcag gagaagatgg aactgaccag taccccttca
      421 caggaatcag caggaggaga tcttcagtct cctttaacac cagaaagtat caatgggaca
      481 gatgatgaga gaacacccga tgtgacacag aactcagagc caagggctga gccagctcag
      541 aatgcattgc cattttcaca tagtgccggg gatcgaaccc agggcctctc tcatgctagt
      601 tcagccatca gcaaacactg ggaggctgaa ctagccacgc tcaaggggaa caatgccaag
      661 ctcaccgcag cgctgctgga gtccactgcc aacgtgaagc agtggaagca acagctggct
      721 gcctaccagg aggaggcaga gcggctgcac aagcgggtca cggagctgga atgtgttagt
      781 agtcaagcaa acgcggtgca cagccacaag acagagctga gtcagacagt gcaggagctg
      841 gaagagaccc taaaagtaaa ggaagaggaa atagaaagat taaaacaaga aattgataac
      901 gccagagaac ttcaagaaca gagggactct ttgactcaga aactacagga agttgagatt
      961 cgaaataaag acctggaggg gcagctgtcg gagctggagc agcgcctgga gaagagccag
     1021 agcgagcagg acgctttccg cagtaacctg aagactctcc tagagattct ggacgggaaa
     1081 atatttgaac taacagaatt gcgggataat ttggccaagc tactagaatg cagcgggtcc
     1141 agcggttcta gcggctccgg gagtggaggg gatccaccgg tcgccaccat ggtgagcaag
     1201 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1261 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1321 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1381 ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     1441 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1501 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1561 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1621 aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg
     1681 aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1741 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1801 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1861 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc ggccgcgact
     1921 ctagatcata atcagccata ccacaatttg taggaggta
//
