LOCUS       sequence-84294-e        2214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2214)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ILK-N-17
FEATURES             Location/Qualifiers
     source          1..2214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1449..1458
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1455..1457,1458..1460,1461..2174)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1500..1521)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1761..1780)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2108..2129
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gaccgccatg gacgacattt
       61 tcactcagtg ccgggagggc aacgcagtcg ccgttcgcct gtggctggac aacacggaga
      121 acgacctcaa ccagggggac gatcatggct tctccccctt gcactgggcc tgccgagagg
      181 gccgctctgc tgtggttgag atgttgatca tgcggggggc acggatcaat gtaatgaacc
      241 gtggggatga cacccccctg catctggcag ccagtcatgg acaccgtgat attgtacaga
      301 agctattgca gtacaaggca gacatcaatg cagtgaatga acatgggaat gtgcccctgc
      361 actatgcctg tttttggggc caagatcaag tggcagagga cctggtggca aatggggccc
      421 ttgtcagcat ctgtaacaag tatggagaga tgcctgtgga caaagccaag gcacccctga
      481 gagagcttct ccgagagcgg gcagagaaga tgggccagaa tctcaaccgt attccataca
      541 aggacacatt ctggaagggg accacccgca ctcggccccg aaatggaacc ctgaacaaac
      601 actctggcat tgacttcaaa cagcttaact tcctgacgaa gctcaacgag aatcactctg
      661 gagagctatg gaagggccgc tggcagggca atgacattgt cgtgaaggtg ctgaaggttc
      721 gagactggag tacaaggaag agcagggact tcaatgaaga gtgtccccgg ctcaggattt
      781 tctcgcatcc aaatgtgctc ccagtgctag gtgcctgcca gtctccacct gctcctcatc
      841 ctactctcat cacacactgg atgccatatg gatccctcta caatgtacta catgaaggca
      901 ccaatttcgt cgtggaccag agccaggctg tgaagtttgc tttggacatg gcaaggggca
      961 tggccttcct acacacacta gagcccctca tcccacgaca tgcactcaat agccgtagtg
     1021 taatgattga tgaggacatg actgcccgaa ttagcatggc tgatgtcaag ttctctttcc
     1081 aatgtcctgg tcgcatgtat gcacctgcct gggtagcccc cgaagctctg cagaagaagc
     1141 ctgaagacac aaacagacgc tcagcagaca tgtggagttt tgcagtgctt ctgtgggaac
     1201 tggtgacacg ggaggtaccc tttgctgacc tctccaatat ggagattgga atgaaggtgg
     1261 cattggaagg ccttcggcct accatcccac caggtatttc ccctcatgtg tgtaagctca
     1321 tgaagatctg catgaatgaa gaccctgcaa agcgacccaa atttgacatg attgtgccta
     1381 tccttgagaa gatgcaggac aagggaattc tgcagtcgac ggtaccgcgg gcccgggatc
     1441 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1501 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1561 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1621 tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc gcccgctacc
     1681 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1741 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1801 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1861 acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg
     1921 acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca
     1981 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2041 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     2101 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2161 agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac attt
//
