LOCUS       sequence-84316-e        2053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Planchon TA, Gao L, Milkie DE, Davidson MW, Galbraith JA, Galbraith 
            CG, Betzig E
  TITLE     Rapid three-dimensional isotropic imaging of living cells using 
            Bessel beam plane illumination.
  JOURNAL   Nat Methods. 2011 May;8(5):417-23. doi: 10.1038/nmeth.1586. Epub 
            2011 Mar 4.
  PUBMED    21378978
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1586"; journalName: "Nat Methods"; date: "2011-05"; 
            volume: "8"; issue: "5"; pages: "417-23"
COMMENT     Sequence Label: mEmerald-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..2053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(37..39,40..42,43..753)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(82..103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(343..362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     690..711
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggacggtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
       61 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc
      241 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      481 cacaaggtct atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc
      541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
      661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      721 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctgg tggctccgca
      781 ggatcccggc aagaagaagc aaaggctgct gtaggtgtga ctggaaatga catcactacc
      841 ccgccaaaca aggagccacc accaagccca gaaaagaaag caaagccttt ggccaccact
      901 caacctgcaa agacttcaac atcgaaagcc aaaacacagc ccacttctct ccctaagcaa
      961 ccagctccca ccacctctgg tgggttgaat aaaaaaccca tgagcctcgc ctcaggctca
     1021 gtgccagctg ccccacacaa acgccctgct gctgccactg ctactgccag gccttccacc
     1081 ctacctgcca gagacgtgaa gccaaagcca attacagaag ctaaggttgc cgaaaagcgg
     1141 acctctccat ccaagccttc atctgcccca gccctcaaac ctggacctaa aaccacccca
     1201 accgtttcaa aagccacatc tccctcaact cttgtttcca ctggaccaag tagtagaagt
     1261 ccagctacaa ctctgcctaa gaggccaacc agcatcaaga ctgaggggaa acctgctgat
     1321 gtcaaaagga tgactgctaa gtctgcctca gctgacttga gtcgctcaaa gaccacctct
     1381 gccagttctg tgaagagaaa caccactccc actggggcag cacccccagc agggatgact
     1441 tccactcgag tcaagcccat gtctgcacct agccgctctt ctggggctct ttctgtggac
     1501 aagaagccca cttccactaa gcctagctcc tctgctccca gggtgagccg cctggccaca
     1561 actgtttctg cccctgacct gaagagtgtt cgctccaagg tcggctctac agaaaacatc
     1621 aaacaccagc ctggaggagg ccgggccaag gtagagaaaa aaacagaggc agctaccaca
     1681 gctgggaagc ctgaacctaa tgcagtcact aaagcagccg gctccattgc gagtgcacag
     1741 aaaccgcctg ctgggaaagt ccagatagta tccaaaaaag tgagctacag tcatattcaa
     1801 tccaagtgtg tttccaagga caatattaag catgtccctg gatgtggcaa tgttcagatt
     1861 cagaacaaga aagtggacat atccaaggtc tcctccaagt gtgggtccaa agctaatatc
     1921 aagcacaagc ctggtggagg agatgtcaag attgaaagtc agaagttgaa cttcaaggag
     1981 aaggcccaag ccaaagtggg atagggatcc accggatcta gataactgat cataatcagc
     2041 cataccacat ttg
//
