LOCUS       sequence-84337-e        3093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3093)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-MPO-N-18
FEATURES             Location/Qualifiers
     source          1..3093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2333..2342
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2339..2341,2342..2344,2345..3058)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2384..2405)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2645..2664)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2992..3013
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttaccgcca tgggggttcc
       61 cttcttctct tctctcagat gcatggtgga cttaggacct tgctgggctg ggggtctcac
      121 tgcagagatg aagctgcttc tggccctagc agggctcctg gccattctgg ccacgcccca
      181 gccctctgaa ggtgctgctc cagctgtcct gggggaggtg gacacctcgt tggtgctgag
      241 ctccatggag gaggccaagc agctggtgga caaggcctac aaggagcggc gggaaagcat
      301 caagcagcgg cttcgcagcg gctcagccag ccccatggaa ctcctatcct acttcaagca
      361 gccggtggca gccaccagga cggcggtgag ggccgctgac tacctgcacg tggctctaga
      421 cctgctggag aggaagctgc ggtccctgtg gcgaaggcca ttcaatgtca ctgatgtgct
      481 gacgcccgcc cagctgaatg tgttgtccaa gtcaagcggc tgcgcctacc aggacgtggg
      541 ggtgacttgc ccggagcagg acaaataccg caccatcacc gggatgtgca acaacagacg
      601 cagccccacg ctgggggcct ccaaccgtgc ctttgtgcgc tggctgccgg cggagtatga
      661 ggacggcttc tctcttccct acggctggac gcccggggtc aagcgcaacg gcttcccggt
      721 ggctctggct cgcgcggtct ccaacgagat cgtgcgcttc cccactgatc agctgactcc
      781 ggaccaggag cgctcactca tgttcatgca atggggccag ctgttggacc acgacctcga
      841 cttcacccct gagccggccg cccgggcctc cttcgtcact ggcgtcaact gcgagaccag
      901 ctgcgttcag cagccgccct gcttcccgct caagatcccg cccaatgacc cccgcatcaa
      961 gaaccaagcc gactgcatcc cgttcttccg ctcctgcccg gcttgccccg ggagcaacat
     1021 caccatccgc aaccagatca acgcgctcac ttccttcgtg gacgccagca tggtgtacgg
     1081 cagcgaggag cccctggcca ggaacctgcg caacatgtcc aaccagctgg ggctgctggc
     1141 cgtcaaccag cgcttccaag acaacggccg ggccctgctg ccctttgaca acctgcacga
     1201 tgacccctgt ctcctcacca accgctcagc gcgcatcccc tgcttcctgg caggggacac
     1261 ccgttccagt gagatgcccg agctcacctc catgcacacc ctcttacttc gggagcacaa
     1321 ccggctggcc acagagctca agagcctgaa ccctaggtgg gatggggaga ggctctacca
     1381 ggaagcccgg aagatcgtgg gggccatggt ccagatcatc acttaccggg actacctgcc
     1441 cctggtgctg gggccaacgg ccatgaggaa gtacctgccc acgtaccgtt cctacaatga
     1501 ctcagtggac ccacgcatcg ccaacgtctt caccaatgcc ttccgctacg gccacaccct
     1561 catccaaccc ttcatgttcc gcctggacaa tcggtaccag cccatggaac ccaacccccg
     1621 tgtccccctc agcagggtct tttttgcctc ctggagggtc gtgctggaag gtggcattga
     1681 ccccatcctc cggggcctca tggccacccc tgccaagctg aatcgtcaga accaaattgc
     1741 agtggatgag atccgggagc gattgtttga gcaggtcatg aggattgggc tggacctgcc
     1801 tgctctgaac atgcagcgca gcagggacca cggcctccca ggatacaatg cctggaggcg
     1861 cttctgtggg ctcccgcagc ctgaaactgt gggccagctg ggcacggtgc tgaggaacct
     1921 gaaattggcg aggaaactga tggagcagta tggcacgccc aacaacatcg acatctggat
     1981 gggcggcgtg tccgagcctc tgaagcgcaa aggccgcgtg ggcccactcc tcgcctgcat
     2041 catcggtacc cagttcagga agctccggga tggtgatcgg ttttggtggg agaacgaggg
     2101 tgtgttcagc atgcagcagc gacaggccct ggcccagatc tcattgcccc ggatcatctg
     2161 cgacaacaca ggcatcacca ccgtgtctaa gaacaacatc ttcatgtcca actcatatcc
     2221 ccgggacttt gtcaactgca gtacacttcc tgcattgaac ctggcttcct ggagggaagc
     2281 ctccgggaga ggcagcggga gtggcagtcg cggaggcagt ggtggaccgg tcgccaccat
     2341 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2401 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     2461 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2521 cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     2581 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2641 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2701 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2761 gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg
     2821 catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2881 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2941 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     3001 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
     3061 ggccgcgact ctagatcata atcagccata cca
//
