LOCUS       sequence-84362-e        2505 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2505)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Paxillin-KM-C-10
FEATURES             Location/Qualifiers
     source          1..2505
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctggcag cagtggaggt
      781 gacgacctcg atgccttact ggcagacctg gaatccacca cctcgcatat ctctaagcga
      841 ccggtgtttc taacagagga aacgccttac tcctacccaa ctggaaacca tacgtaccag
      901 gagattgctg tgccaccgcc ggtgcccccg ccgccttcca gtgaggccct aaatggcact
      961 gtgattgacc ccttagacca gtggcaaccc agcgtgtcca gatacggcca ccagcaacct
     1021 cagtcccagt ctccgatata cagctctagt gccaaaagct ccagtgcctc tgttcctaga
     1081 gacgggctca gctccccttc tccccgtgcc agtgaggagg aacacgtgta cagcttccca
     1141 aacaagcaga agtctgcaga accatctccc tcactgacca gcacctccct gggcagcaac
     1201 ctctcggaac tggacaggct tctcctggaa ctgaatgctg ttcaacataa tccccccagt
     1261 ggcttctcag cagatgaagt cagcagaagc ccatcactgc ccaatgtgac cggacctcac
     1321 tatgtcatcc cagagagcag cagctctgca ggagggaagg ctgcaccccc tacaaaagaa
     1381 aaaccaaagc ggaatggtgg gcgtgggatt gaagatgtgc gtcccagtgt ggagagcctg
     1441 ctggatgagc tggagagctc tgtgccaagc ccagtccctg caatcactgt gagccaaggg
     1501 gaggtgagca gcccccagcg tgtcaacgcc agtcagcagc agacccgtat ctctgcttct
     1561 tcagctacac gagaactgga tgagctgatg gcgtccctct ctgactttaa gttcatggca
     1621 caggggaaag ccggcggcag cagctcccca ccatccacca ccccaaagcc tggcagtcag
     1681 ctggacacca tgctgggaag tctgcagtct gacctgaaca aactgggggt agcgacggtc
     1741 gccaagggtg tgtgcggagc ctgcaagaag ccgattgctg ggcaggtagt tacagccatg
     1801 gggaagacct ggcaccctga acacttcgtc tgcacccact gccaggagga gattggatca
     1861 cggaacttct ttgagcggga tgggcagccc tactgtgaga aggactatca caacctcttc
     1921 tctcctcgct gctactactg caatgggcca atccttgata aagtggtgac ggctttggac
     1981 aggacgtggc accctgaaca ctttttctgt gcccagtgtg gagttttctt tggacctgaa
     2041 ggatttcatg agaaggatgg caaagcctat tgccgcaagg actactttga catgtttgct
     2101 cccaagtgtg gaggctgtgc ccgggctatc ctggaaaatt acatttctgc cttaaacacc
     2161 ctgtggcacc ctgagtgctt tgtctgtcgg gaatgtttca caccgttcat caacggcagc
     2221 ttcttcgagc acgacgggca gccctactgc gaggtgcatt accacgagcg ccgcggctcg
     2281 ctgtgctctg gctgccagaa gcccatcacg ggacgctgca tcactgccat gggcaagaaa
     2341 tttcaccccg aacactttgt ctgtgccttc tgcctcaagc agctcaacaa aggaaccttc
     2401 aaagaacaga acgacaagcc ctactgtcag aactgctttc tcaaactctt ctgttaagga
     2461 tccaccggat ctagataact gatcataatc agccatacca cattt
//
