LOCUS       sequence-84372-e        2243 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2243)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Peripherin-N-18
FEATURES             Location/Qualifiers
     source          1..2243
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1486..1495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1492..1494,1495..1497,1498..2211)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1537..1558)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1798..1817)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2145..2166
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgttcagat tccggctagc agccaccatg agccaccacc cgtcgggcct ccgggccggc
       61 ttcagctcca cctcataccg ccgtaccttc ggtccaccgc cctcactatc ccccggggcc
      121 ttctcctact cgtccagctc ccgcttctcc agcagccgcc tgctgggctc cgcgtccccg
      181 agctcctcgg tgcgcctggg cagcttccgt agcccccgag cgggagcggg cgccctcctg
      241 cgcctgccct cggagcgcct cgacttctcc atggccgagg ccctcaacca ggagttcctg
      301 gccacgcgca gcaacgagaa gcaggagctg caggagctca acgaccgctt cgccaacttc
      361 atcgagaagg tacgctttct ggagcagcag aacgcggccc tgcgcgggga gctgagccaa
      421 gcccggggcc aggagccggc gcgcgccgac cagctgtgcc agcaggagct gcgcgagctg
      481 cggcgagagc tggagctgtt gggccgcgag cgtgaccggg tgcaggtgga gcgcgacggg
      541 ctggcggagg acctggcggc gctcaagcag aggttggagg aggagacgcg caagcgggag
      601 gacgcggagc acaacctcgt gctcttccgc aaggacgtgg acgatgccac tctgtcccgc
      661 ctggaactag agcgcaagat tgagtctctg atggatgaga ttgagttcct caagaagctg
      721 cacgaggagg agctgcgaga cctgcaggtg agtgtggaga gccagcaggt gcagcaggtg
      781 gaggtggaag ccacggtgaa gcccgagctg acggcagcgc tgagggacat ccgcgcgcag
      841 tacgagagca tcgccgcgaa gaacctgcag gaggcggagg agtggtacaa gtccaagtac
      901 gcggacctgt ccgacgctgc caaccggaac cacgaggccc tgcgccaggc caagcaggag
      961 atgaacgagt cccgacgcca gatccagagt ctaacgtgcg aggtggacgg gctgcgcggc
     1021 acgaacgagg cgctgctcag gcagttgaga gagctggagg agcagttcgc cctggaggcg
     1081 gggggctacc aggcgggcgc tgcgcggctc gaggaggagc tgcgacagct aaaagaggag
     1141 atggcgcggc acctgaggga gtaccaggag ctcctcaacg tcaagatggc cctggacatc
     1201 gagatcgcca cctaccgcaa gctgctggag ggcgaggaga gccggatctc cgtgcccgtc
     1261 cattcttttg cctccttaaa tataaagacg actgtgcctg aggtggagcc tccccaggac
     1321 agccacagcc ggaagacggt tctgatcaag accattgaga cccggaatgg ggaggtggtg
     1381 acagagtccc agaaggagca gcgcagtgag ctggacaagt cttctgccca cagttacagc
     1441 ggtgggagcg gtggcgcatc cggcggtagc ggggatccac cggtcgccac catggtgagc
     1501 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1561 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1621 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1681 accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     1741 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1801 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1861 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1921 tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag
     1981 gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2041 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2101 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2161 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgcg
     2221 actctagatc ataatcagcc ata
//
