LOCUS       sequence-84383-e        3038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3038)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-PSD95-C-16
FEATURES             Location/Qualifiers
     source          1..3038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(29..31,32..34,35..745)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(74..95)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(335..354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     682..703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2966..3018
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
       61 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg
      241 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt
      481 ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa
      541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      601 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
      661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      721 tctcggcatg gacgagctgt acaagtccgg agggagcggc agtgggtcca gtggagggag
      781 cagtggaagc ggagactgtc tctgtatagt gacaaccaag aaataccgct accaagatga
      841 agacacgccc cctctggaac acagcccggc ccacctcccc aaccaggcca attctccccc
      901 tgtgattgtc aacacggaca ccctagaagc cccaggatat gagttgcagg tgaatggaac
      961 agagggggag atggagtatg aggagatcac attggaaagg ggtaactcag gtctgggctt
     1021 cagcatcgca ggtggcactg acaacccgca catcggtgac gacccgtcca tttttatcac
     1081 caagatcatt cctggtgggg ctgcagccca ggatggccgc ctcagggtca atgacagcat
     1141 cctgtttgta aatgaagtgg atgttcggga ggtgacccat tcagctgcgg tggaggccct
     1201 caaagaggca ggttccatcg ttcgcctcta tgtcatgcgc cggaaacccc cagccgaaaa
     1261 ggtcatggag atcaaactca tcaaagggcc taaaggactt ggcttcagca ttgcgggggg
     1321 cgttgggaac cagcacatcc ctggagataa cagcatctat gtaacgaaga tcatcgaagg
     1381 aggtgctgcc cacaaggatg gcaggttgca gattggagac aagatcctgg cggtcaacag
     1441 tgtggggctg gaggacgtca tgcacgagga tgccgtggca gccctgaaga acacatatga
     1501 cgttgtgtac ctaaaggtgg ccaagcccag caatgcctac ctgagtgaca gctatgctcc
     1561 cccagacatc acaacctcgt attctcagca cctggacaat gagatcagtc atagcagcta
     1621 cttgggcact gactacccca cagccatgac ccccacttcc cctcggcgct actcccctgt
     1681 ggccaaggac ctgctggggg aggaagacat tccccgggaa ccaaggcgga tcgtgatcca
     1741 tcggggctcc accggcctgg gcttcaacat cgtgggcggc gaggatggtg aaggcatctt
     1801 catctccttc atccttgctg ggggtccagc cgacctcagt ggggagctac ggaaggggga
     1861 ccagatcctg tcggtcaatg gtgttgacct ccgcaatgcc agtcacgaac aggctgccat
     1921 tgccctgaag aatgcgggtc agacggtcac gatcatcgct cagtataaac cagaagagta
     1981 tagtcgattc gaggccaaga tccatgatct tcgggaacag ctcatgaata gtagcctagg
     2041 ctcagggact gcatccttgc gaagcaaccc caagaggggc ttctacatta gggccctgtt
     2101 tgattacgac aagaccaagg actgcggttt cttgagccag gccctgagct tccgcttcgg
     2161 ggatgtgctt catgtcattg acgctggtga cgaagagtgg tggcaagcac ggcgggtcca
     2221 ctccgacagt gagaccgacg acattggctt cattcccagc aaacggcggg tcgagcgacg
     2281 agagtggtca aggttaaagg ccaaggactg gggctccagc tctggatcac agggtcgaga
     2341 agactcggtt ctgagctatg agacggtgac ccagatggaa gtgcactatg ctcgtcccat
     2401 catcatcctt ggacccacca aagaccgtgc caacgatgat cttctctccg agttccccga
     2461 caagtttgga tcctgtgtcc ctcatacgac acgtcctaag cgggaatatg agatagacgg
     2521 ccgggattac cactttgtct cctcccggga gaaaatggag aaggacatcc aggcacacaa
     2581 gttcattgag gctggccagt acaacagcca cctctatggg accagcgtcc agtctgtgcg
     2641 agaggtagca gagcagggga agcactgcat cctcgatgtc tcggccaatg ccgtgcggcg
     2701 gctgcaggcg gcccacctgc accccatcgc catcttcatc cgtccccgct ccctggagaa
     2761 tgtgctagag atcaataagc ggatcacaga ggagcaagcc cggaaagcct tcgacagagc
     2821 cacgaagctg gagcaggagt tcacagagtg cttctcagcc atcgtagagg gcgacagctt
     2881 tgaagagatc tatcacaaag tgaaacgtgt cattgaagac ctctcaggcc cctacatctg
     2941 ggtcccagcc cgagagagac tctgactcga gctcaagctt cgaattctgc agtcgacggt
     3001 accgcgggcc cgggatccac cggatctaga taactgat
//
