LOCUS       sequence-84400-e        1587 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Kinetochore, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1587)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Ska1-C-18
FEATURES             Location/Qualifiers
     source          1..1587
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(26..28,29..31,32..742)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(71..92)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(332..351)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     679..700
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
       61 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      121 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      181 caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt
      241 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      301 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      361 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      421 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta
      481 tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat
      541 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      601 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
      661 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      721 cggcatggac gagctgtaca agtccggaag tgctggcagc ggtggaggca gcgcatccgg
      781 cggaggcagc ggaagcgcct cgtcagatct ggaacaatta tgctctcatg ttaatgaaaa
      841 gattggcaat attaagaaaa ccttatcatt aagaaactgt ggccaggaac ctaccttgaa
      901 aactgtatta aataaaatag gagatgagat cattgtaata aatgaacttc taaataaatt
      961 ggaattggaa attcagtatc aagaacaaac caacaattca ctcaaggaac tctgtgaatc
     1021 tcttgaagaa gattacaaag acatagaaca tcttaaagaa aacggtcctt cccatttgcc
     1081 tcaagtaaca gtaacccaga gctgtgttaa gggatcagat cttgatcctg aagaaccaat
     1141 caaagttgaa gaacctgaac ccgtaaagaa gcctcccaaa gagcaaagaa gtattaagga
     1201 aatgccattt ataacttgtg atgagttcaa tggtgttcct tcgtacatga aatcccgctt
     1261 aacctataat caaattaatg atgttattaa agaaatcaac aaggcagtaa ttagtaaata
     1321 taaaatccta catcagccaa aaaagtctat gaattctgtg accagaaatc tctatcacag
     1381 atttattgat gaagaaacga aggataccaa aggtcgttat tttatagtgg aagctgacat
     1441 aaaggagttc acaactttga aagctgacaa gaagtttcgc gtgttactga atattttacg
     1501 acactgccgg aggctatcag aggtccgagg gggaggactt actcgttatg ttataacctg
     1561 aggatccacc ggatctagat aactgat
//
