LOCUS       sequence-84403-e        1721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1721)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Stomatin-N-16
FEATURES             Location/Qualifiers
     source          1..1721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      953..962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(959..961,962..964,965..1678)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1004..1025)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1265..1284)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1612..1633
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag gccaccatgg ccgagaagcg
       61 gcacacacgg gactccgaag cccagcggct ccccgactcc ttcaaggaca gccccagtaa
      121 gggccttgga ccttgcggat ggattttggt ggcgttctca ttcttattca ccgttataac
      181 tttcccaatc tcaatatgga tgtgcataaa gattataaaa gagtatgaaa gagccatcat
      241 ctttagattg ggtcgcattt tacaaggagg agccaaagga cctggtttgt tttttattct
      301 gccatgcact gacagcttca tcaaagtgga catgagaact atttcatttg atattcctcc
      361 tcaggagatc ctcacaaagg attcagtgac aattagcgtg gatggtgtgg tctattaccg
      421 cgttcagaat gcaaccctgg ctgtggcaaa tatcaccaac gctgactcag caacccgtct
      481 tttggcacaa actactctga ggaatgttct gggcaccaag aatctttctc agatcctctc
      541 tgacagagaa gaaattgcac acaacatgca gtctactctg gatgatgcca ctgatgcctg
      601 gggaataaag gtggagcgtg tggaaattaa ggatgtgaaa ctacctgtgc agctccagag
      661 agctatggct gcagaagcag aagcgtcccg cgaggcccgc gccaaggtta ttgcagccga
      721 aggagaaatg aatgcatcca gggctctgaa agaagcctcc atggtcatca ctgaatctcc
      781 tgcagccctt cagctccgat acctgcagac actgaccacc attgctgctg agaaaaactc
      841 aacaattgtc ttccctctgc ccatagatat gctgcaagga atcatagggg caaaacacag
      901 ccatctaggc gcatccgggg gtgcgtcagg cggcagcgga tctggaccgg tcgccaccat
      961 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1021 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1081 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1141 cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     1201 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1261 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1321 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1381 gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg
     1441 catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1501 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1561 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1621 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
     1681 ggccgcgact ctagatcata atcagccata ccacattgta g
//
