LOCUS       sequence-84407-e        8469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Paszek MJ, DuFort CC, Rubashkin MG, Davidson MW, Thorn KS, Liphardt 
            JT, Weaver VM
  TITLE     Scanning angle interference microscopy reveals cell dynamics at the 
            nanoscale.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):825-7. doi: 10.1038/nmeth.2077.
  PUBMED    22751201
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "825-7"
COMMENT     Sequence Label: mEmerald-Talin-N-10
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7704..7713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7710..7712,7713..7715,7716..8429)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7755..7776)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8016..8035)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8363..8384
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcta gccaccatgg
       61 ttgcgctttc gctgaagatt agcattggga atgtggtgaa gacgatgcaa tttgagccat
      121 ctaccatggt gtacgacgcc tgccgcatga ttcgtgagcg gatcccagag gccctggctg
      181 gccctcccaa cgactttggg ctctttctgt cagatgatga ccccaaaaaa ggcatctggc
      241 tagaggctgg gaaagctttg gactactaca tgctccgaaa tggggacacc atggagtaca
      301 gaaaaaagca gagacccctg aagatacgga tgttagacgg aacggtgaag actatcatgg
      361 tggatgactc caagaccgtc acagacatgc tcatgaccat ttgtgcccga attggtatca
      421 ccaaccatga tgagtattca ctggttcgag agctgatgga agaaaagaaa gatgagggga
      481 cagggaccct gagaaaagac aagaccctac tgcgagatga aaagaagatg gagaagctaa
      541 agcagaagtt gcacacagac gatgagttga actggctgga ccatgggcgg acactgaggg
      601 aacagggagt ggaagagcat gagacgttgc tgctgcggag aaagttcttc tactcagacc
      661 agaatgtgga ttcccgagac cctgtacagc tgaaccttct ctatgtgcag gcacgagatg
      721 acatcctgaa tggctcccat cctgtctcct ttgacaaggc ctgtgaattt gcaggcttcc
      781 agtgccagat ccagtttgga cctcacaatg aacagaagca caaggctggc ttccttgacc
      841 tgaaggactt cctgcccaag gagtatgtga agcagaaggg agagcgtaag atctttcagg
      901 cacacaagaa ttgtgggcag atgagtgaaa ttgaggccaa ggtacgctac gtgaagctgg
      961 cccgttccct caagacttac ggtgtctcct tcttcctagt caaggaaaag atgaagggga
     1021 agaataaact ggtgcccagg ctgttgggca tcactaagga gtgtgtgatg cgtgtggatg
     1081 agaagaccaa ggaggtgatt caggaatgga gcctcaccaa catcaaacgc tgggctgcct
     1141 ctcccaagag ctttactctg gactttggag actatcagga tggctactac tcagtacaga
     1201 cgacagaagg cgagcagatc gcacagctca ttgctggcta catagatatc atccttaaga
     1261 agaaaaaaag caaggaccat tttgggctgg agggagatga agagtctact atgttggagg
     1321 actcagtgtc tcccaaaaag tcaacagtcc ttcagcagca gtacaaccga gtggggaaag
     1381 tggagcacgg ctctgtggct ctgccagcca tcatgcgctc tggagcctct ggtcctgaga
     1441 atttccaggt ggggagcatg ccacctgccc agcagcagat caccagtggc cagatgcacc
     1501 gaggacacat gccacctctg acttcagccc agcaggcgct cactggaacc attaactcca
     1561 gcatgcaggc tgtgcaggct gcccaggcca ctctggatga ctttgagact ctaccccctc
     1621 ttggccagga tgctgcctcc aaagcttggc gtaagaacaa gatggatgaa tcaaagcatg
     1681 agatccactc ccaggtagat gccatcacag ctggcactgc ctctgtggta aacctgacag
     1741 caggagatcc tgcagagaca gactatacag cagtgggctg tgcagtcacc accatctctt
     1801 ccaacctgac ggagatgtcc cgtggggtga agctattggc tgccctgctg gaggatgaag
     1861 gcggcaatgg ccggcccctt ctgcaagcag caaagggcct tgcaggggct gtgtcggaac
     1921 tgcttcgcag tgctcagccc gccagcgctg agcctcgtca gaacctgctg caggcagccg
     1981 ggaacgtggg ccaggccagt ggggagctgt tgcagcaaat tggggaaagt gacactgacc
     2041 cccacttcca ggatgttcta atgcagctag caaacgcagt ggcaagtgct gcagccgccc
     2101 tggtcctcaa ggccaagagt gtggcccagc gcacagagga ttccgggctt cagacccaag
     2161 tgattgctgc agctacgcaa tgtgctctgt ccacttccca gctggtggcc tgtaccaagg
     2221 tggtagcacc tacaatcagc tcacctgtgt gccaagaaca gctagtcgag gctgggcgac
     2281 tggtggccaa agctgtggag ggctgtgtgt ctgcctcgca agcagctaca gaagatggac
     2341 agctgcttcg aggggtagga gcagcagcca cggctgtcac ccaggccctc aatgagctgc
     2401 tgcagcacgt gaaggcccac gccaccggag ctgggcctgc cggtcggtat gaccaagcca
     2461 ctgataccat cctcaccgtc actgaaaaca tcttcagctc catgggtgat gctggggaga
     2521 tggtgcgcca ggcccgcatc ctggcccaag ccacctcaga cctggtcaat gctatcaagg
     2581 ccgatgctga gggggagagt gatctggaga actctagaaa gctcctgagt gctgccaaga
     2641 tcctcgctga tgccaccgcc aagatggtgg aggcggccaa gggagcagcc gcccaccctg
     2701 acagtgagga acagcagcag cgactgcgtg aagcagctga ggggcttcgc atggccacca
     2761 atgcagctgc gcagaacgcc atcaagaaga agttggtgca gcgcctggag catgcagcca
     2821 agcaagctgc agcctctgca acacagacca ttgctgcagc ccaacatgca gcctctgccc
     2881 ccaaggcctc tgcgggcccc cagcccctac tggtgcagag ctgtaaggcc gtggcagagc
     2941 agattccgtt gctggtgcag ggtgtccgag gaagtcaagc tcagcctgac agccctagtg
     3001 ctcagctcgc cctcattgct gccagccaga gcttcctgca gccaggtggg aagatggtgg
     3061 cagcggcaaa agcctcagtg ccaacaattc aggaccaggc ttcagcgatg cagctgagtc
     3121 agtgcgcaaa gaacctaggc actgccctgg ccgagctgcg cactgctgct cagaaggctc
     3181 aggaggcatg tggacctttg gagatggatt ctgcactgag tgttgtacaa aatctagaga
     3241 aagacctaca ggaaataaaa gcagcagctc gagatggcaa gcttaagccc ttacctgggg
     3301 aaacaatgga gaagtgtacc caagatcttg gcaacagcac caaagcggtg agctctgcca
     3361 tcgccaagct gctgggggag attgcccagg gcaacgagaa ctatgcaggt attgcagctc
     3421 gggatgtagc tggtggacta aggtcactag cccaggctgc gcggggtgtg gctgcgctga
     3481 catcagatcc tgcagtgcag gccattgtgc ttgacacagc cagcgacgtt ctggacaagg
     3541 ccagcagcct cattgaagag gcaaaaaaag catctggaca cccaggggac ccagaaagcc
     3601 agcaaaggct tgctcaggta gctaaagcag tgacccaagc cctgaaccgc tgtgtcagct
     3661 gcctgcctgg ccaaagagac gtggataatg ccctacgagc cgttggagat gccagcaagc
     3721 gactcctgag tgacttgctt cctccaagca cggggacatt tcaagaagca cagagccgat
     3781 tgaatgaagc tgcagctggg ttaaatcagg ccgccacaga actggtgcaa gcctcccgag
     3841 gaactcctca ggacctggct cgggcctccg gccgatttgg acaggacttc agcaccttcc
     3901 tggaagctgg tgtggagatg gccggacagg ccccgagcca ggaggaccga gcccaggtgg
     3961 tgtccaacct gaagggcata tccatgtctt caagcaaact tctccttgcc gctaaggcct
     4021 tgtccacaga ccctgcttct cccaacctca agagtcagct ggctgcggct gcccgggcag
     4081 tgacggacag catcaaccag ctcatcacca tgtgcaccca gcaggcacct ggccagaagg
     4141 agtgtgacaa tgcacttcgg cagctagaga cagtccgaga actcctggag aatccagtcc
     4201 agcccatcaa cgacatgtcc tacttcggtt gcttggacag tgtcatggag aactccaagg
     4261 tcctaggtga ggccatgact ggcatctccc aaaatgccaa gaatggaaat ctgccggagt
     4321 ttggagatgc cattgccaca gcctccaagg ctctctgtgg cttcacggag gcagccgcac
     4381 aggcagcata tctagttggt gtctctgacc ccaacagcca agctggacag caaggactgg
     4441 tggaacccac acagtttgcc cgtgcaaacc aggcaattca gatggcctgt cagagtctgg
     4501 gggagcctgg ctgtacccag gcccaggtgt tatctgcagc cactattgta gccaaacaca
     4561 catctgcatt gtgtaacagc tgtcgcctgg cttccgctag gactgccaat cccactgcca
     4621 agcgccagtt tgtacagtca gccaaggagg tggccaacag tacagccaat cttgtcaaga
     4681 ccatcaaggc actagatggg gacttcacag aagagaaccg tgcccagtgc cgagcagcca
     4741 cagcccctct gctggaagct gtggataacc tgagtgcctt tgcctccaac cctgagttct
     4801 ccagcgtccc tgcccagatc agccctgagg gcagagcagc catggagccc attgtaatct
     4861 ctgctaagac aatgttggag agtgctggag ggctcatcca gacagctcgg gcgttagcag
     4921 tcaatccccg agacccccca cgttggtctg tgctagctgg ccactctcgg actgtctcag
     4981 attccatcaa gaaacttatt acaagcatga gggacaaagc cccagggcag ctggagtgtg
     5041 agacagccat tgcggctctg aacagctgct tgcgggacct agatcaggct tcgcttgccg
     5101 ctgtcagcca gcagcttgct ccccgtgaag gaatctctca agaggctttg cacacccaga
     5161 tgctcactgc agtgcaagaa atttctcatc tcattgagcc gctggccagc gctgctcggg
     5221 ctgaagcctc ccagctggga cacaaggtgt cccaaatggc ccagtacttt gaaccactca
     5281 ccctggctgc agtgggtgct gcgtctaaga ccctgagcca cccgcagcag atggcacttc
     5341 tggaccagac taaaacgttg gcagagtctg ccttgcagtt gctatatact gccaaggagg
     5401 ctggtggtaa ccccaagcaa gcagcacaca cccaggaagc cctggaggag gctgtgcaga
     5461 tgatgacaga ggccgtagaa gacctgacga cgaccctcaa cgaagcagcc agtgctgcag
     5521 gagtcgttgg cggcatggtg gactctatca ctcaggccat caaccagcta gatgaaggac
     5581 ctatgggtga cccagaaggc tcattcgtag attaccagac aaccatggtg aggacagcca
     5641 aggccattgc tgtcactgtt caggagatgg taaccaagtc aaacaccagc cctgaagagc
     5701 taggccctct tgccaaccag ctgaccagtg actatggccg actggcctca caagccaagc
     5761 ctgcagctgt ggctgctgaa aatgaagaga taggcgctca catcaaacac cgagtacagg
     5821 agctgggcca tggctgctct gctctggtca ccaaggcagg tgccttgcag tgtagcccca
     5881 gtgatgtcta caccaagaag gagctcatag agtgtgcccg cagagtgtca gagaaggtct
     5941 cccatgttct ggctgcactc caggctggga atcgtggtac ccaggcctgc attacagcag
     6001 ccagtgctgt gtctggtatc attgctgacc tcgacaccac catcatgttc gctactgctg
     6061 gcacacttaa ccgtgagggt gctgaaactt ttgctgacca ccgggagggt atcttaaaga
     6121 cagcaaaggt tcttgtggag gacaccaagg tcctagtgca gaatgcagct gggagccagg
     6181 agaagttggc acaagccgcc cagtcctccg tggccaccat tacccgcctc gctgatgtgg
     6241 tcaagctcgg tgcagccagc ctaggagccg aagaccctga aactcaggtg gtgctgatca
     6301 atgcagtaaa ggacgtagcc aaggccctgg gtgacctcat cagcgctacg aaggctgcag
     6361 cgggcaaagt tggggatgac cctgcagtgt ggcagctcaa gaactctgcc aaggtgatgg
     6421 tgaccaatgt gacatcattg ctcaagacag tgaaggctgt ggaagatgag gccaccaaag
     6481 gcacacgggc cctagaggca accacagagc acatacgtca ggaactggcg gtcttctgtt
     6541 ccccagagcc acctgccaag acctctaccc ctgaagactt catccgaatg accaagggta
     6601 ttactatggc aacagccaaa gccgttgctg ctggcaattc ctgtcgacag gaagatgtca
     6661 ttgccacagc caatctgagc cgacgggcta ttgcggacat gcttcgggct tgcaaggaag
     6721 cagctttcca cccagaagtg gcacctgatg tacggctccg agccctgcac tatggccggg
     6781 agtgtgccaa tggttacctg gaactgctgg accacgtgct gctgaccctt cagaagccaa
     6841 acccagacct gaagcagcag ctaactggac actcaaagcg ggttgctggc tctgtgactg
     6901 agctcatcca ggctgctgaa gccatgaaag gaacagagtg ggtggaccca gaggacccta
     6961 ctgtcattgc tgagaatgag ctcttgggag ccgcagccgc catcgaggct gcagccaaga
     7021 agctggagca gctgaagccc agggccaaac ccaaggaggc ggatgagtcc ttgaactttg
     7081 aggaacaaat cctagaagct gccaagtcca tcgctgcagc caccagtgca ctggtaaagg
     7141 ctgcgtcagc agcccagagg gagctggtgg ctcaaggaaa ggtgggcgcc attccagcca
     7201 atgcactgga tgatgggcag tggtcgcagg gcctcatttc cgctgcccgt atggtggctg
     7261 cggccaccaa caatctgtgt gaagcagcta acgcagctgt ccagggccat gctagccagg
     7321 agaaactcat ttcctcagcc aagcaagtag ctgcctccac agctcagctc ctggtagctt
     7381 gcaaggtcaa agcagatcag gactctgagg caatgaaacg gctccaggct gctggcaatg
     7441 cagtgaagag ggcctcagat aacctggtaa aggcggccca gaaagctgca gccttcgaag
     7501 accaggagaa tgagacggtg gtggtgaagg agaaaatggt tgggggcatt gcccagatta
     7561 tcgcagcaca ggaagagatg cttcggaagg aacgagagct ggaagaggct cggaaaaagc
     7621 tcgcccagat ccggcagcag cagtacaagt tcttgccttc agagcttcga gacgagcacg
     7681 gctccgcgtc ccgatcaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     7741 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     7801 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     7861 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt
     7921 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     7981 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     8041 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     8101 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg
     8161 tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca
     8221 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     8281 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
     8341 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     8401 ctctcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat
     8461 accacattt
//
