LOCUS       sequence-84414-e        6017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6017)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Tensin1-C-14
FEATURES             Location/Qualifiers
     source          1..6017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt
      241 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg
      481 tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca
      541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
      661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      721 ctctcggcat ggacgagctg tacaagtccg gaagtgctgg gagcgcaagc ggaagcggag
      781 gctccggtag tgtgagccgg accatggagg acagctgtga gctggacctg gtgtacgtca
      841 cagagaggat catcgctgtc tccttcccca gcacagccaa tgaggagaac tttcggagca
      901 acctccgtga ggtggcgcag atgctcaagt ccaaacatgg aggcaactac ctgctgttca
      961 acctctctga gcggagacct gacatcacga agctccatgc caaggtactg gaatttggct
     1021 ggcccgacct ccacacccca gccctggaga agatatgcag catctgtaag gccatggaca
     1081 catggctcaa tgcagaccct cacaatgtcg ttgttctaca caacaaggga aaccgaggca
     1141 ggataggagt tgtcatcgcg gcttacatgc actacagcaa catttctgcc agtgcggacc
     1201 aggctctgga tcggtttgca atgaagcggt tctatgagga taagattgtg cccattggcc
     1261 agccatccca aagaaggtac gtgcattact tcagtggcct gctctccggc tccatcaaaa
     1321 tgaacaacaa gcccttgttt ctgcaccacg tgatcatgca cggcatcccc aactttgagt
     1381 ctaaaggagg atgtcggcca tttctccgca tctaccaggc catgcaacct gtgtacacat
     1441 ctggcatcta caacatccca ggagacagcc agactagcgt ctgcatcacc atcgagccag
     1501 gactgctctt gaagggagac atcttgctga agtgctacca caagaagttc cgaagcccag
     1561 cccgagacgt catcttccgt gtgcagttcc acacctgtgc catccatgac ctgggggttg
     1621 tctttgggaa ggaggacctt gatgatgctt tcaaagatga tcgatttcca gagtatggca
     1681 aagtggagtt tgtattttct tatgggccag agaaaattca aggcatggag cacctggaga
     1741 acgggccgag cgtgtctgtg gactataaca cctccgaccc cctcatccgc tgggactcct
     1801 acgacaactt cagtgggcat cgagatgacg gcatggagga ggtggtggga cacacgcagg
     1861 ggccactaga tgggagcctg tatgctaagg tgaagaagaa agactccctg cacggcagca
     1921 ccggggctgt taatgccaca cgtcctacac tgtcggccac ccccaaccac gtggaacaca
     1981 cgctttctgt gagcagcgac tcgggcaact ccacagcctc caccaagacc gacaagaccg
     2041 acgagcctgt ccccggggcc tccagtgcca ctgctgcctt gagtccccag gagaagcggg
     2101 agctggaccg cctgctgagt ggctttggct tagagcgaga gaagcaaggc gccatgtacc
     2161 acacccagca cctcaggtcc cgcccagcag ggggctcggc tgtgccctcc tctggacgcc
     2221 acgttgtccc agcccaggtt catgtcaatg gtggggcgtt agcatctgag cgggagacag
     2281 acatcctgga cgatgaattg ccaaaccagg atggtcacag tgcgggcagc atgggcacac
     2341 tctcttctct ggacggggtc accaacacca gtgagggggg ctacccagag gccctgtccc
     2401 cactgaccaa cggtctggac aagtcctacc ccatggagcc tatggtcaat ggaggaggct
     2461 acccctacga gtctgccagc cgggcggggc ctgcccatgc tggccacacg gcccccatgc
     2521 ggccctccta ctctgcacag gagggtttag ctggctacca gagggagggg ccccacccag
     2581 cctggccaca gccagtgacc acctcccact atgcccatga ccccagcggt atgttccgct
     2641 ctcaatcctt ttcggaagct gaaccccagc tgcccccagc tccggtccga gggggaagca
     2701 gccgggaggc tgtgcaaagg ggactgaact cgtggcagca gcagcagcag cagcagcagc
     2761 agcctcgccc acctccacgc cagcaggaaa gagcccactt ggagagtctt gtagccagca
     2821 ggcccagccc tcagccattg gcagagaccc ccatccccag tctccctgag ttcccgcgag
     2881 cagcctccca gcaggagatt gaacagtcca tcgaaacact caatatgctg atgctggacc
     2941 tggagccagc ctccgctgct gccccactac acaagtccca gagtgtcccc ggggcctggc
     3001 caggggcttc tccactctcc tcccagcccc tctctgggtc ctcccgtcag tcccatccac
     3061 tgacccagtc cagatatggc tatatcccca gtgggcattc gttgggaacc cctgagccag
     3121 ccccacgggc ctctctggag tctgtccctc ctggcaggtc ttactcacct tatgactatc
     3181 agccatgttt ggctgggcct aaccaggatt tccattcaaa gagcccagcc tcttcctcct
     3241 tgcctgcctt ccttccgacc acccacagcc ctccagggcc tcagcaaccc ccagcctctc
     3301 tccctggcct cactgctcag cctctgctct caccaaagga agcgacttca gacccctccc
     3361 ggactccaga ggaggagcca ttgaatttag aagggctggt ggcccacagg gtagcagggg
     3421 tacaggctcg ggagaagcag cctgcagagc ccccagcccc tctgcggagg cgggcggcca
     3481 gtgatggaca gtatgagaac cagtctccag aagccacatc ccctcgtagc cctggggttc
     3541 gctcccctgt ccagtgtgtc tccccggagc tggctcttac catcgctctc aatcctggag
     3601 ggcggcccaa agagccccat ttgcacagct acaaggaggc cttcgaggag atggagggaa
     3661 cctccccgag cagcccacca cccagtgggg tgcggtcccc cccgggtctg gccaagacac
     3721 ccctgtctgc tctgggcctg aaacctcaca acccagcgga catcctgttg caccccacag
     3781 gagttaccag aagacggatt cagccagagg aagatgaggg gaaggtggtg gtcaggctgt
     3841 cagaagagcc ccggagctat gtggagtctg tggcacggac agcggtggct ggaccccgag
     3901 ctcaggactc tgagcccaag agctttagtg ctccagccac ccaggcctat ggccatgaga
     3961 tacccctgag gaacgggacc ctgggtggct cctttgtctc ccccagcccc ctctccacca
     4021 gcagccccat cctcagtgct gacagcactt cagtggggag tttcccgtcg ggagagagca
     4081 gtgaccaggg tccccggacg cccacccagc ctctgttgga gtctggcttc cgctcaggca
     4141 gcctgggaca gcccagcccg tctgcccaga gaaactacca gagctcttct cctctcccga
     4201 ctgtgggcag tagctacagc agccccgact actcacttca gcatttcagc tcctctccag
     4261 aaagccaggc tcgcgctcag ttcagtgtgg ctggcgtcca cacggtgcct gggagccctc
     4321 aggcgcgcca cagaacagtg ggcaccaaca ctccccctag tcctggcttc ggctggcggg
     4381 ccatcaatcc cagcatggct gcccccagca gtcccagttt gagccatcac cagatgatgg
     4441 gtccaccagg cactggcttc catggtagca ctgtctccag cccccagagc agtgcagcga
     4501 ccaccccggg gagccccagc ctgtgtcggc acccagcagg ggtctaccag gtttctggcc
     4561 tccacaacaa agtggccacc accccgggga gtcccagcct gggccggcac cctggggctc
     4621 accaaggcaa cctggcctcc ggtcttcata gcaatgcaat agccagccct ggaagcccca
     4681 gcctgggccg tcacctcgga gggtctggat ctgtggttcc cggcagcccc tgcttggacc
     4741 ggcatgtggc ctatggtggc tattctaccc cggaggatcg gagacccaca ctgtcccggc
     4801 agagcagtgc ctctggctac caggctcctt ccacgccctc cttccctgtc tcccctgcct
     4861 actaccctgg cctgagcagc cctgccacct ccccgtcacc agactccgca gccttccggc
     4921 aagggagccc aacaccagcc ttgccagaga agcgaaggat gtcagtggga gaccgggcag
     4981 gcagcctccc caactatgcc accatcaatg ggaaggtgtc ttcgcctgtc gccagcggca
     5041 tgtccagtcc cagtgggggc agcaccgtct ccttctccca cactctgccc gacttctcca
     5101 agtactccat gccagacaac agcccggaga cgcgggctaa agtgaagttt gtccaggaca
     5161 cttctaagta ttggtacaag cctgagattt ccagggagca ggccatcgcg ctcctcaagg
     5221 accaggagcc gggggccttc atcatccgcg acagtcactc cttccgaggc gcgtacgggc
     5281 tggccatgaa ggtgtcttcg ccacctccaa ccatcatgca gcagaataaa aaaggagaca
     5341 tgacccatga gctggtcagg cattttctga tagagactgg ccccagagga gtcaagctca
     5401 agggctgccc caatgagcca aacttcggat cgctgtctgc cctggtctac cagcactcca
     5461 tcatcccatt ggccctgcct tgcaagctgg tcattccaaa ccgagacccc acagatgaat
     5521 cgaaagatag ctccggccct gccaactcaa ctgcagacct gctgaaacaa ggggcagcct
     5581 gcaatgtgct cttcgtcaac tctgtggaca tggagtcact cactgggcca caggccatct
     5641 ctaaagccac atctgagacg ttggctgcag accccacgcc agctgccacc atcgttcact
     5701 tcaaagtctc tgcccaggga atcactctga ctgacaacca gagaaagctc tttttcagac
     5761 gccactaccc tctcaacact gtcaccttct gtgacctgga cccacaggaa agaaagtgga
     5821 tgaaaacaga gggtggtgcc cctgctaagc tcttcggctt cgtggcccgg aagcagggca
     5881 gcaccacgga caacgcctgc cacctctttg ctgagcttga ccccaaccag ccggcctctg
     5941 ccatcgtcaa cttcgtctcc aaggtcatgc tgaatgccgg ccaaaagaga tgaggatcca
     6001 ccggatctag ataactg
//
