LOCUS       sequence-84415-e        6047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6047)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Tensin1-N-18
FEATURES             Location/Qualifiers
     source          1..6047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5286..5295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5292..5294,5295..5297,5298..6011)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5337..5358)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5598..5617)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5945..5966
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcgctacc ggactcagat ctcgaggcca ccatgagtgt gagccggacc atggaggaca
       61 gctgtgagct ggacctggtg tacgtcacag agaggatcat cgctgtctcc ttccccagca
      121 cagccaatga ggagaacttt cggagcaacc tccgtgaggt ggcgcagatg ctcaagtcca
      181 aacatggagg caactacctg ctgttcaacc tctctgagcg gagacctgac atcacgaagc
      241 tccatgccaa ggtactggaa tttggctggc ccgacctcca caccccagcc ctggagaaga
      301 tatgcagcat ctgtaaggcc atggacacat ggctcaatgc agaccctcac aatgtcgttg
      361 ttctacacaa caagggaaac cgaggcagga taggagttgt catcgcggct tacatgcact
      421 acagcaacat ttctgccagt gcggaccagg ctctggatcg gtttgcaatg aagcggttct
      481 atgaggataa gattgtgccc attggccagc catcccaaag aaggtacgtg cattacttca
      541 gtggcctgct ctccggctcc atcaaaatga acaacaagcc cttgtttctg caccacgtga
      601 tcatgcacgg catccccaac tttgagtcta aaggaggatg tcggccattt ctccgcatct
      661 accaggccat gcaacctgtg tacacatctg gcatctacaa catcccagga gacagccaga
      721 ctagcgtctg catcaccatc gagccaggac tgctcttgaa gggagacatc ttgctgaagt
      781 gctaccacaa gaagttccga agcccagccc gagacgtcat cttccgtgtg cagttccaca
      841 cctgtgccat ccatgacctg ggggttgtct ttgggaagga ggaccttgat gatgctttca
      901 aagatgatcg atttccagag tatggcaaag tggagtttgt attttcttat gggccagaga
      961 aaattcaagg catggagcac ctggagaacg ggccgagcgt gtctgtggac tataacacct
     1021 ccgaccccct catccgctgg gactcctacg acaacttcag tgggcatcga gatgacggca
     1081 tggaggaggt ggtgggacac acgcaggggc cactagatgg gagcctgtat gctaaggtga
     1141 agaagaaaga ctccctgcac ggcagcaccg gggctgttaa tgccacacgt cctacactgt
     1201 cggccacccc caaccacgtg gaacacacgc tttctgtgag cagcgactcg ggcaactcca
     1261 cagcctccac caagaccgac aagaccgacg agcctgtccc cggggcctcc agtgccactg
     1321 ctgccttgag tccccaggag aagcgggagc tggaccgcct gctgagtggc tttggcttag
     1381 agcgagagaa gcaaggcgcc atgtaccaca cccagcacct caggtcccgc ccagcagggg
     1441 gctcggctgt gccctcctct ggacgccacg ttgtcccagc ccaggttcat gtcaatggtg
     1501 gggcgttagc atctgagcgg gagacagaca tcctggacga tgaattgcca aaccaggatg
     1561 gtcacagtgc gggcagcatg ggcacactct cttctctgga cggggtcacc aacaccagtg
     1621 aggggggcta cccagaggcc ctgtccccac tgaccaacgg tctggacaag tcctacccca
     1681 tggagcctat ggtcaatgga ggaggctacc cctacgagtc tgccagccgg gcggggcctg
     1741 cccatgctgg ccacacggcc cccatgcggc cctcctactc tgcacaggag ggtttagctg
     1801 gctaccagag ggaggggccc cacccagcct ggccacagcc agtgaccacc tcccactatg
     1861 cccatgaccc cagcggtatg ttccgctctc aatccttttc ggaagctgaa ccccagctgc
     1921 ccccagctcc ggtccgaggg ggaagcagcc gggaggctgt gcaaagggga ctgaactcgt
     1981 ggcagcagca gcagcagcag cagcagcagc ctcgcccacc tccacgccag caggaaagag
     2041 cccacttgga gagtcttgta gccagcaggc ccagccctca gccattggca gagaccccca
     2101 tccccagtct ccctgagttc ccgcgagcag cctcccagca ggagattgaa cagtccatcg
     2161 aaacactcaa tatgctgatg ctggacctgg agccagcctc cgctgctgcc ccactacaca
     2221 agtcccagag tgtccccggg gcctggccag gggcttctcc actctcctcc cagcccctct
     2281 ctgggtcctc ccgtcagtcc catccactga cccagtccag atatggctat atccccagtg
     2341 ggcattcgtt gggaacccct gagccagccc cacgggcctc tctggagtct gtccctcctg
     2401 gcaggtctta ctcaccttat gactatcagc catgtttggc tgggcctaac caggatttcc
     2461 attcaaagag cccagcctct tcctccttgc ctgccttcct tccgaccacc cacagccctc
     2521 cagggcctca gcaaccccca gcctctctcc ctggcctcac tgctcagcct ctgctctcac
     2581 caaaggaagc gacttcagac ccctcccgga ctccagagga ggagccattg aatttagaag
     2641 ggctggtggc ccacagggta gcaggggtac aggctcggga gaagcagcct gcagagcccc
     2701 cagcccctct gcggaggcgg gcggccagtg atggacagta tgagaaccag tctccagaag
     2761 ccacatcccc tcgtagccct ggggttcgct cccctgtcca gtgtgtctcc ccggagctgg
     2821 ctcttaccat cgctctcaat cctggagggc ggcccaaaga gccccatttg cacagctaca
     2881 aggaggcctt cgaggagatg gagggaacct ccccgagcag cccaccaccc agtggggtgc
     2941 ggtccccccc gggtctggcc aagacacccc tgtctgctct gggcctgaaa cctcacaacc
     3001 cagcggacat cctgttgcac cccacaggag ttaccagaag acggattcag ccagaggaag
     3061 atgaggggaa ggtggtggtc aggctgtcag aagagccccg gagctatgtg gagtctgtgg
     3121 cacggacagc ggtggctgga ccccgagctc aggactctga gcccaagagc tttagtgctc
     3181 cagccaccca ggcctatggc catgagatac ccctgaggaa cgggaccctg ggtggctcct
     3241 ttgtctcccc cagccccctc tccaccagca gccccatcct cagtgctgac agcacttcag
     3301 tggggagttt cccgtcggga gagagcagtg accagggtcc ccggacgccc acccagcctc
     3361 tgttggagtc tggcttccgc tcaggcagcc tgggacagcc cagcccgtct gcccagagaa
     3421 actaccagag ctcttctcct ctcccgactg tgggcagtag ctacagcagc cccgactact
     3481 cacttcagca tttcagctcc tctccagaaa gccaggctcg cgctcagttc agtgtggctg
     3541 gcgtccacac ggtgcctggg agccctcagg cgcgccacag aacagtgggc accaacactc
     3601 cccctagtcc tggcttcggc tggcgggcca tcaatcccag catggctgcc cccagcagtc
     3661 ccagtttgag ccatcaccag atgatgggtc caccaggcac tggcttccat ggtagcactg
     3721 tctccagccc ccagagcagt gcagcgacca ccccggggag ccccagcctg tgtcggcacc
     3781 cagcaggggt ctaccaggtt tctggcctcc acaacaaagt ggccaccacc ccggggagtc
     3841 ccagcctggg ccggcaccct ggggctcacc aaggcaacct ggcctccggt cttcatagca
     3901 atgcaatagc cagccctgga agccccagcc tgggccgtca cctcggaggg tctggatctg
     3961 tggttcccgg cagcccctgc ttggaccggc atgtggccta tggtggctat tctaccccgg
     4021 aggatcggag acccacactg tcccggcaga gcagtgcctc tggctaccag gctccttcca
     4081 cgccctcctt ccctgtctcc cctgcctact accctggcct gagcagccct gccacctccc
     4141 cgtcaccaga ctccgcagcc ttccggcaag ggagcccaac accagccttg ccagagaagc
     4201 gaaggatgtc agtgggagac cgggcaggca gcctccccaa ctatgccacc atcaatggga
     4261 aggtgtcttc gcctgtcgcc agcggcatgt ccagtcccag tgggggcagc accgtctcct
     4321 tctcccacac tctgcccgac ttctccaagt actccatgcc agacaacagc ccggagacgc
     4381 gggctaaagt gaagtttgtc caggacactt ctaagtattg gtacaagcct gagatttcca
     4441 gggagcaggc catcgcgctc ctcaaggacc aggagccggg ggccttcatc atccgcgaca
     4501 gtcactcctt ccgaggcgcg tacgggctgg ccatgaaggt gtcttcgcca cctccaacca
     4561 tcatgcagca gaataaaaaa ggagacatga cccatgagct ggtcaggcat tttctgatag
     4621 agactggccc cagaggagtc aagctcaagg gctgccccaa tgagccaaac ttcggatcgc
     4681 tgtctgccct ggtctaccag cactccatca tcccattggc cctgccttgc aagctggtca
     4741 ttccaaaccg agaccccaca gatgaatcga aagatagctc cggccctgcc aactcaactg
     4801 cagacctgct gaaacaaggg gcagcctgca atgtgctctt cgtcaactct gtggacatgg
     4861 agtcactcac tgggccacag gccatctcta aagccacatc tgagacgttg gctgcagacc
     4921 ccacgccagc tgccaccatc gttcacttca aagtctctgc ccagggaatc actctgactg
     4981 acaaccagag aaagctcttt ttcagacgcc actaccctct caacactgtc accttctgtg
     5041 acctggaccc acaggaaaga aagtggatga aaacagaggg tggtgcccct gctaagctct
     5101 tcggcttcgt ggcccggaag cagggcagca ccacggacaa cgcctgccac ctctttgctg
     5161 agcttgaccc caaccagccg gcctctgcca tcgtcaactt cgtctccaag gtcatgctga
     5221 atgccggcca aaagagaggt agcgcatccg ggggtgcgtc aggcggcagc ggatctggac
     5281 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     5341 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     5401 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     5461 cctggcccac cctcgtgacc accttgacct acggcgtgca gtgcttcgcc cgctaccccg
     5521 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     5581 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     5641 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5701 tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca
     5761 agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg
     5821 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5881 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     5941 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6001 tgtacaagta agcggccgcg actctagatc ataatcagcc ataccac
//
