LOCUS       sequence-84425-e        1677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1677)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Tropomyosin-N-14
FEATURES             Location/Qualifiers
     source          1..1677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      913..922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(919..921,922..924,925..1638)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(964..985)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1225..1244)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1572..1593
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatggac gccatcaaga agaagatgca gatgctgaag
       61 ctcgacaagg agaacgcctt ggatcgagct gagcaggcgg aggccgacaa gaaggcggcg
      121 gaagacagga gcaagcagct ggaagatgag ctggtgtcac tgcaaaagaa actcaagggc
      181 accgaagatg aactggacaa atactctgag gctctcaaag atgcccagga gaagctggag
      241 ctggcagaga aaaaggccac cgatgctgaa gccgacgtag cttctctgaa cagacgcatc
      301 cagctggttg aggaagagtt ggatcgtgcc caggagcgtc tggcaacagc tttgcagaag
      361 ctggaggaag ctgagaaggc agcagatgag agtgagagag gcatgaaagt cattgagagt
      421 cgagcccaaa aagatgaaga aaaaatggaa attcaggaga tccaactgaa agaggcaaag
      481 cacattgctg aagatgccga ccgcaaatat gaagaggtgg cccgtaagct ggtcatcatt
      541 gagagcgacc tggaacgtgc agaggagcgg gctgagctct cagaaggcaa atgtgccgag
      601 cttgaagaag aattgaaaac tgtgacgaac aacttgaagt cactggaggc tcaggctgag
      661 aagtactcgc agaaggaaga cagatatgag gaagagatca aggtcctttc cgacaagctg
      721 aaggaggctg agactcgggc tgagtttgcg gagaggtcag taactaaatt ggagaaaagc
      781 attgatgact tagaagacga gctgtacgct cagaaactga agtacaaagc catcagcgag
      841 gagctggacc acgctctcaa cgatatgact tccatatccg gcggtagcgg cggtagcggg
      901 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      961 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1021 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1081 cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc
     1141 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1201 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1261 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1321 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc
     1381 gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac
     1441 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1501 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
     1561 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1621 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatt
//
