LOCUS       sequence-84449-e        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  full-length native Gal4 with stop in Gateway middle entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL
  TITLE     Gal80 intersectional regulation of cell-type specific expression in 
            vertebrates.
  JOURNAL   Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 
            2011 Sep 8.
  PUBMED    21905164
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.22734"; journalName: "Dev Dyn"; date: "2011-10"; 
            volume: "240"; issue: "10"; pages: "2324-34"
COMMENT     Sequence Label: pME-Gal4
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..124
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(126..470)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 ctatagggga tatcagctgg atggcaaata atgattttat tttgactgat agtgacctgt
       61 tcgttgcaac aaattgataa gcaatgcttt cttataatgc caactttgta caagaaagct
      121 gggtcttact ctttttttgg gtttggtggg gtatcttcat catcgaatag atagttatat
      181 acatcatcca ttgtagtggt attaaacatc cctgtagtga ttccaaacgc gttatacgca
      241 gtttggtccg tccaaccagg tgacagtggt tttgaattat taccatcatc aattttacta
      301 gccgtgattt cattattcat gaagttatca tgaacgttag aggaggcaat tggttgtgaa
      361 agcgcttgag aatttgtttg agttgttatg aggttcggac cgttgctact gttagtgaaa
      421 gtgaaggaca atgagctatc agcaatattc ccactttgat taaaattggc gccaccaaac
      481 aaagcagacg gggtcagtgg cactaatgat tgcagctgtt gctgttgccc tagaaaaggc
      541 gtgactgagc gatgcgaagg tgtgcttctt ggtattgtca ctggagagtt acgagagggt
      601 ggacggttag ataacagctt gactagatca ctgaaacttg ctcctgattt caatggcaca
      661 ggtgaaggcc ctactgagcc aggagaaaca tatttaacac tgatattgtt gacattttcc
      721 tccggaagag tagggtattg ggcgatagtt gcagaaccga caatattttt aatggcgcta
      781 ccattactat tgttataact gatatgcggt aatgggattg cacactgtga taacagaaac
      841 ggcgcacata cctcttccag tacttgaatg tatttttcac aagtctggat tttaaaagtg
      901 gccagttttt ttaatagcat cagaacagtg ttaatttgtt gtaataattg tgcggtctcg
      961 ttattctcag cattcgattt tgagtttgag agtagagtct ttatgggtac taggactgca
     1021 ttgaacaagt aataagaaca attccaggca aaatatgggg tgacattatg attgtccata
     1081 tagctactta cagacataac agttctttgt gctgcatcgc ttaacatgat ggagcatcgt
     1141 ttaacttcat aactttgatg atcattt
//
