LOCUS       sequence-84454-e        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hFMRP iso 1 I304N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ascano M Jr, Mukherjee N, Bandaru P, Miller JB, Nusbaum JD, Corcoran
            DL, Langlois C, Munschauer M, Dewell S, Hafner M, Williams Z, Ohler 
            U, Tuschl T
  TITLE     FMRP targets distinct mRNA sequence elements to regulate protein 
            expression.
  JOURNAL   Nature. 2012 Dec 20;492(7429):382-6. doi: 10.1038/nature11737. Epub 
            2012 Dec 12.
  PUBMED    23235829
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11737"; journalName: "Nature"; date: "2012-12-20- 
            20"; volume: "492"; issue: "7429"; pages: "382-6"
COMMENT     Sequence Label: pFRT-TODestFLAGHAhFMRPiso1I304N
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             191..214
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             215..241
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    257..281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      181 tggtaccatg gactacaagg acgacgatga caagtaccct tatgacgtgc ccgattacgc
      241 tttgaaccag atatcaacaa gtttgtacaa aaaagcaggc tccaccatgg aggagctggt
      301 ggtggaagtg cggggctcca atggcgcttt ctacaaggca tttgtaaagg atgttcatga
      361 agattcaata acagttgcat ttgaaaacaa ctggcagcct gataggcaga ttccatttca
      421 tgatgtcaga ttcccacctc ctgtaggtta taataaagat ataaatgaaa gtgatgaagt
      481 tgaggtgtat tccagagcaa atgaaaaaga gccttgctgt tggtggttag ctaaagtgag
      541 gatgataaag ggtgagtttt atgtgataga atatgcagca tgtgatgcaa cttacaatga
      601 aattgtcaca attgaacgtc taagatctgt taatcccaac aaacctgcca caaaagatac
      661 tttccataag atcaagctgg atgtgccaga agacttacgg caaatgtgtg ccaaagaggc
      721 ggcacataag gattttaaaa aggcagttgg tgccttttct gtaacttatg atccagaaaa
      781 ttatcagctt gtcattttgt ccatcaatga agtcacctca aagcgagcac atatgctgat
      841 tgacatgcac tttcggagtc tgcgcactaa gttgtctctg ataatgagaa atgaagaagc
      901 tagtaagcag ctggagagtt caaggcagct tgcctcgaga tttcatgaac agtttatcgt
      961 aagagaagat ctgatgggtc tagctattgg tactcatggt gctaatattc agcaagctag
     1021 aaaagtacct ggggtcactg ctattgatct agatgaagat acctgcacat ttcatattta
     1081 tggagaggat caggatgcag tgaaaaaagc tagaagcttt ctcgaatttg ctgaagatgt
     1141 aatacaagtt ccaaggaact tagtaggcaa agtaatag
//
