LOCUS       sequence-84467-e        1121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hFMRP iso 1 I304N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ascano M Jr, Mukherjee N, Bandaru P, Miller JB, Nusbaum JD, Corcoran
            DL, Langlois C, Munschauer M, Dewell S, Hafner M, Williams Z, Ohler 
            U, Tuschl T
  TITLE     FMRP targets distinct mRNA sequence elements to regulate protein 
            expression.
  JOURNAL   Nature. 2012 Dec 20;492(7429):382-6. doi: 10.1038/nature11737. Epub 
            2012 Dec 12.
  PUBMED    23235829
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11737"; journalName: "Nature"; date: "2012-12-20- 
            20"; volume: "492"; issue: "7429"; pages: "382-6"
COMMENT     Sequence Label: pFRT-TODestFLAGHAhFMRPiso1I304N
FEATURES             Location/Qualifiers
     source          1..1121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    36..260
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     249..266
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(346..387)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    440..464
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc
       61 atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag
      121 aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac
      181 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
      241 ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctctagactc
      301 gaggcacact ggatcagtta tctatgcggc cgcggatcag gtacgcgtag aatcgagacc
      361 gaggagaggg ttagggatag gcttaccttc gaaccgcggg ccctctagac ttgagcggcc
      421 gccactgtgc tggatatcaa ccactttgta caagaaagct gggtctagat atctcgagtg
      481 cggccgcgaa ttcgtttagg gtactccatt cacgagtggt tgctgaccat ccacgctgtc
      541 tggcttctct ttcttctggt tacgatcttt acccgtgcgc agccgactac cttcactgga
      601 ggtattctgt aatgttttag tgtggacact cctttcatta ttgcagtcaa ctctgatctg
      661 aagggatcca tctgttgttc ttcctttagc ctctcttgga ttacgtggac gattatctgt
      721 tcgggagtga tcgtcgtttc ctttgaagcc tcctccacgt cctcttcctc cttgtcctct
      781 tcctccccct ccacgccgcc gtccgtctcc tctgcgcagg aagctctccc tctcttcctc
      841 tgttggagct aatgaccaat cactgagttc gtctctgtgg tcagattctg tttcagaagc
      901 atttgatgct tcagaattag ttcctgaagt atatccagga ccgcgtctgc cgtgccccct
      961 atttctgtaa ggtctactac ctcgacccat tccttgacca tcatcagtca catagctttt
     1021 ttccttatct gtacgatttg gtggtggtct agaactagct ccaatctgtc gcaactgctc
     1081 atcaatttgt aatctctcca aacgcaactg gtctacttcc t
//
