LOCUS       sequence-84502-e        1188 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hFXR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ascano M Jr, Mukherjee N, Bandaru P, Miller JB, Nusbaum JD, Corcoran
            DL, Langlois C, Munschauer M, Dewell S, Hafner M, Williams Z, Ohler 
            U, Tuschl T
  TITLE     FMRP targets distinct mRNA sequence elements to regulate protein 
            expression.
  JOURNAL   Nature. 2012 Dec 20;492(7429):382-6. doi: 10.1038/nature11737. Epub 
            2012 Dec 12.
  PUBMED    23235829
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11737"; journalName: "Nature"; date: "2012-12-20- 
            20"; volume: "492"; issue: "7429"; pages: "382-6"
COMMENT     Sequence Label: pFRT-TODestFLAGHAhFXR1
FEATURES             Location/Qualifiers
     source          1..1188
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             191..214
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             215..241
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    257..281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      181 tggtaccatg gactacaagg acgacgatga caagtaccct tatgacgtgc ccgattacgc
      241 tttgaaccag atatcaacaa gtttgtacaa aaaagcaggc tccaccatgg gaaccaattc
      301 agtcgacatg gcggagctga cggtggaggt tcgcggctct aacggggctt tctacaaggg
      361 atttatcaaa gatgttcatg aagactccct tacagttgtt tttgaaaata attggcaacc
      421 agaacgccag gttccattta atgaagttag attaccacca ccacctgata taaaaaaaga
      481 aattagtgaa ggagatgaag tagaggtata ttcaagagca aatgaccaag agccatgtgg
      541 gtggtggttg gctaaagttc ggatgatgaa aggagaattt tatgtcattg aatatgctgc
      601 ttgtgacgct acttacaatg aaatagtcac atttgaacga cttcggcctg tcaatcaaaa
      661 taaaactgtc aaaaaaaata ccttctttaa atgcacagtg gatgttcctg aggatttgag
      721 agaggcgtgt gctaatgaaa atgcacataa agattttaag aaagcagtag gagcatgcag
      781 aattttttac catccagaaa caacacagct aatgatactg tctgccagtg aagcaactgt
      841 gaagagagta aacatcttaa gtgacatgca tttgcgaagt attcgtacga agttgatgct
      901 tatgtccaga aatgaagagg ccactaagca tttagaatgc acaaaacaac ttgcagcagc
      961 ttttcatgag gaatttgttg tgagagaaga tttaatgggc ctggcaatag gaacacatgg
     1021 tagtaacatc cagcaagcta ggaaggttcc tggagttacc gccattgagc tagatgaaga
     1081 tactggaaca ttcagaatct acggagagag tgctgatgct gtaaaaaagg ctagaggttt
     1141 cttggaattt gtggaggatt ttattcaggt tcctaggaat ctcgttgg
//
