LOCUS       sequence-84515-e        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hFXR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ascano M Jr, Mukherjee N, Bandaru P, Miller JB, Nusbaum JD, Corcoran
            DL, Langlois C, Munschauer M, Dewell S, Hafner M, Williams Z, Ohler 
            U, Tuschl T
  TITLE     FMRP targets distinct mRNA sequence elements to regulate protein 
            expression.
  JOURNAL   Nature. 2012 Dec 20;492(7429):382-6. doi: 10.1038/nature11737. Epub 
            2012 Dec 12.
  PUBMED    23235829
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11737"; journalName: "Nature"; date: "2012-12-20- 
            20"; volume: "492"; issue: "7429"; pages: "382-6"
COMMENT     Sequence Label: pFRT-TODestFLAGHAhFXR2
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             191..214
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             215..241
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    257..281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      181 tggtaccatg gactacaagg acgacgatga caagtaccct tatgacgtgc ccgattacgc
      241 tttgaaccag atatcaacaa gtttgtacaa aaaagcaggc tccaccatgg gaaccaattc
      301 agtcgacatg ggcggcctgg cctctggggg ggatgtggag ccgggactgc ccgtcgaggt
      361 gcgcggctcc aacggggcct tctacaaggg ctttgtgaag gatgtccatg aagactctgt
      421 caccatcttc tttgaaaaca actggcagag tgagagacaa attccttttg gggatgtccg
      481 gctaccacct ccagctgact ataataagga gatcacagaa ggggatgaag tggaggttta
      541 ttctcgagcc aatgaacagg aaccttgtgg ctggtggctg gcccgggtgc ggatgatgaa
      601 gggagatttc tatgtcattg aatatgctgc ctgtgatgcc acctacaatg aaattgttac
      661 cctggagcga cttcggccag ttaatcccaa tccccttgca accaaaggca gcttcttcaa
      721 ggttaccatg gctgtgcccg aggatctgag agaagcctgc tccaatgaaa acgtccataa
      781 agagttcaag aaagccctgg gagccaactg catctttctc aacatcacaa acagtgagct
      841 cttcattctg tcaaccacag aagcccctgt gaagcgagca tctctgctgg gtgatatgca
      901 tttccgaagc ctgcgcacca aactgctact tatgtcccgc aatgaagaag ctaccaagca
      961 cctagagaca agcaagcagt tggcagcagc cttccaagag gagttcacag tgcgagagga
     1021 cctgatggga ctggcaattg ggactcacgg tgccaacatc cagcaggccc gaaaagtacc
     1081 tggggtgacc gccattgagt tgggtga
//
