LOCUS       sequence-84663-e        2568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI B-C N-NES KOZ b c7
FEATURES             Location/Qualifiers
     source          1..2568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(525..530,531..547,548..553))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      668..1256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1157..1176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1410..1427
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1544..1565
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1580..1585,1586..1603,1604..1610)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1618..1634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1623..1645
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1642..1658
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1642..1658
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1862..1879)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1862..1878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1871..1893)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2087..2106)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2206..2228
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2266..2284)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(join(2371..2568,1..336))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tgcgagatca tagatataga tctcactacg cggctgctca aacctgggca gaacgtaagc
       61 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
      121 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
      181 gccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
      241 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
      301 gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac
      361 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
      421 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
      481 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
      541 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgc ctcatgacca
      601 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      661 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      721 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      781 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      841 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      901 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      961 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1021 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1081 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1141 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1201 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1261 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1321 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1381 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1441 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1501 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     1561 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     1621 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat
     1681 gcaggcctct gcagtcgacg ggcccgggat ccgatatcta gatgcattcg atggtctcat
     1741 ctgaacaatg ctgcagaacg agcttgctct taagttggct ggacttgata ttaacaagac
     1801 tggaggagtc gactcgagtg cggccgccac ctgagaccta cgaggtaccg agctcgaatt
     1861 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1921 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1981 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     2041 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     2101 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     2161 cttgtctgct cccggcatcc gcttacagac aagctgtgac cttctccggg agctgcatgt
     2221 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagaca aaagggcctc gtgatacgcc
     2281 tatttttata ggttaatgtc atgagcggcg ttgtgacaat ttaccgaaca actccgcggc
     2341 cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa
     2401 gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc
     2461 gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag
     2521 attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagac
//
