LOCUS       sequence-84723-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Cacchiarelli D, Trapnell C, Ziller MJ, Soumillon M, Cesana M, Karnik
            R, Donaghey J, Smith ZD, Ratanasirintrawoot S, Zhang X, Ho Sui SJ, 
            Wu Z, Akopian V, Gifford CA, Doench J, Rinn JL, Daley GQ, Meissner 
            A, Lander ES, Mikkelsen TS
  TITLE     Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of
            Induced Pluripotency.
  JOURNAL   Cell. 2015 Jul 16;162(2):412-24. doi: 10.1016/j.cell.2015.06.016.
  PUBMED    26186193
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.06"; journalName: "Cell"; date: "2015-07-16- 
            16"; volume: "162"; issue: "2"; pages: "412-24"
COMMENT     Sequence Label: FUW-tetO-hOKMS
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..130)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             complement(200..229)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(473..499)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
ORIGIN
        1 tgccgccgcc gccccccgaa gtttgctgcg ggcccggcgg cttcagctcc gtctccatca
       61 tgttgtacat gggaccgggg ttactttcaa catcgccagc gagtttcaac aaagcgtagt
      121 tagtacattg tccagatccc gcacaagagt tccgtagctg ttcaagtttg tgtttcaact
      181 gttctcgtcg tttccgcaac aagtcctctt cagaaatgag cttttgctcc tctgcttgga
      241 cggacaggat gtatgctgtg gcttttttaa ggataactac cttgggggcc ttttcattgt
      301 tttccaactc cgggatctgg tcacgcaggg caaaaaagct ccgttttagc tcgttcctcc
      361 tctggcgctc caagacgttg tgtgttcgcc tcttgacatt ctcctcggtg tccgaggacc
      421 tggggctggt gcattttcgg ttgttgctga tctgtctcag gactctgaca ctgtccaact
      481 tgaccctctt ggcagcagga tagtccttcc gagtggaggg aggcgctgcg tagttgtgct
      541 gatgtgtgga gacgtggcac ctcttgagga ccagtgggct gtgaggaggt ttgctgtggc
      601 ctccagcaga aggtgatcca gactctgacc ttttgccagg agcctgcctc ttttccacag
      661 aaacaacatc gatttcttcc tcatcttctt gttcctcctc agagtcgctg ctggtggtgg
      721 gcggtgtctc ctcatgga
//
