LOCUS       sequence-84805-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty backbone for retroviral gene expression. Select recipient 
            cells using GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     Viral vectors.
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBABE GFP
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(44..64)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        49..378
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      229..364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(387..403)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(388..404)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gtggtggtac gtaggaattc gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta
       61 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      121 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
      181 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
      241 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
      301 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
      361 ggcctaggct tttgcaaaaa gcttatcgat accgtcgacc tcgagaccat ggtgagcaag
      421 cagatcctga agaacaccgg cctgcaggag atcatgagct tcaaggtgaa cctggagggc
      481 gtggtgaaca accacgtgtt caccatggag ggctgcggca agggcaacat cctgttcggc
      541 aaccagctgg tgcagatccg cgtgaccaag ggcgcccccc tgcccttcgc cttcgacatc
      601 ctgagccccg ccttccagta cggcaaccgc accttcacca agtaccccga ggacatcagc
      661 gacttcttca tccagagctt ccccgccggc ttcgtgtacg agcgcaccct gcgctacgag
      721 gacggcggcc tggtggagat ccgcagcgac atcaacctga tcgaggagat gttcgtgtac
      781 cgcgtggagt acaagggccg caacttcccc aacgacggcc ccgtgatgaa gaagaccatc
      841 accggcctgc agcccagctt cgaggtggtg tacatgaacg acggcgtgct ggtgggccag
      901 gtgatcctgg tgtaccgcct gaacagcggc aagttctaca gctgccacat gcgcaccctg
//
