LOCUS       sequence-84848-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Cashikar AG, Schirmer EC, Hattendorf DA, Glover JR, Ramakrishnan MS,
            Ware DM, Lindquist SL
  TITLE     Defining a pathway of communication from the C-terminal peptide 
            binding domain to the N-terminal ATPase domain in a AAA protein.
  JOURNAL   Mol Cell 2002 Apr;9(4):751-60.
  PUBMED    11983167
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2002-04"; volume: "9(4)"; pages: "751-60"
COMMENT     Sequence Label: pJC45BS104 L892W
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             55..84
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             97..108
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat gggccatcat
       61 catcatcatc atcatcatca tcacagcagc ggccatatcg aaggtcgtgg atccgggatg
      121 aacgaccaaa cgcaatttac agaaagggct ctaacgattt tgacgttggc tcaaaaattg
      181 gcttcggatc atcaacatcc acaattacaa cctatacata ttctagctgc cttcattgaa
      241 acgccagaag atggatcagt cccttaccta cagaatctaa ttgagaaggg ccgttacgac
      301 tatgatcttt tcaagaaagt ggttaataga aatctagtaa gaattcctca acagcaacct
      361 gcacctgcgg agataactcc aagttatgct ttggggaaag tccttcaaga cgctgctaag
      421 attcaaaaac aacagaagga ctcatttata gcgcaagacc atatattgtt tgctctattc
      481 aatgattcgt ctattcagca aatatttaag gaagctcaag tagatattga ggccatcaag
      541 caacaagctc ttgaacttcg tggtaacact agaattgact ctcgtggcgc tgatacgaac
      601 acacctttgg aatatttatc
//
