LOCUS       sequence-84875-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  aTc-inducible expression of wild-type Cas9 (S .pyogenes) for 
            bacterial gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.02"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1173-83"
COMMENT     Sequence Label: pwtCas9-bacteria
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        69..124
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    105..123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             142..153
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat cattaattcc
       61 taatttttgt tgacactcta tcgttgatag agttatttta ccactcccta tcagtgatag
      121 agaaaagaat tcaaaagatc taaagaggag aaaggatcta tggataagaa atactcaata
      181 ggcttagata tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
      241 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
      301 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
      361 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
      421 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
      481 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
      541 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
      601 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
      661 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
      721 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
      781 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
      841 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
      901 ttg
//
