LOCUS       sequence-84890-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 558, Emission: 583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DsRed2-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..79
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     30..50
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    102..182
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      184..193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             190..867
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(370..390)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     782..805
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
       61 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca
      121 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
      181 gtcgccacca tggcctcctc cgagaacgtc atcaccgagt tcatgcgctt caaggtgcgc
      241 atggagggca ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      301 tacgagggcc acaacaccgt gaagctgaag gtgaccaagg gcggccccct gcccttcgcc
      361 tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc
      421 gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      481 aacttcgagg acggcggcgt ggcgaccgtg acccaggact cctccctgca ggacggctgc
      541 ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc cgtgatgcag
      601 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
      661 aagggcgaga cccacaaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
      721 aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggacgcc
      781 aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta cgagcgcacc
      841 gagggccgcc accacctgtt cctgtaagcg gccgcgactc tagatcataa tcagccatac
      901 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      961 acataaaatg aatgcaattg ttgttgttaa cttg
//
