LOCUS       sequence-84891-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, 
            Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, 
            Masai H, Miyawaki A
  TITLE     Visualizing spatiotemporal dynamics of multicellular cell-cycle 
            progression.
  JOURNAL   Cell. 2008 Feb 8;132(3):487-98. doi: 10.1016/j.cell.2007.12.033.
  PUBMED    18267078
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2007.12"; journalName: "Cell"; date: "2008-02-8- 8";
            volume: "132"; issue: "3"; pages: "487-98"
COMMENT     Sequence Label: mKO2-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..79
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     30..50
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             124..777
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     misc_feature    778..843
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
       61 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc
      121 accatggtga gtgtgattaa accagagatg aagatgaggt actacatgga cggctccgtc
      181 aatgggcatg agttcacaat tgaaggtgaa ggcacaggca gaccttacga gggacatcaa
      241 gagatgacac tacgcgtcac aatggccgag ggcgggccaa tgcctttcgc gtttgactta
      301 gtgtcacacg tgttctgtta cggccacaga gtatttacta aatatccaga agagatacca
      361 gactatttca aacaagcatt tcctgaaggc ctgtcatggg aaaggtcgtt ggagttcgaa
      421 gatggtgggt ccgcttcagt cagtgcgcat ataagcctta gaggaaacac cttctaccac
      481 aaatccaaat ttactggggt taactttcct gccgatggtc ctatcatgca aaaccaaagt
      541 gttgattggg agccatcaac cgagaaaatt actgccagcg acggagttct gaagggtgat
      601 gttacgatgt acctaaaact tgaaggaggc ggcaatcaca aatgccaaat gaagactact
      661 tacaaggcgg caaaagagat tcttgaaatg ccaggagacc attacatcgg ccatcgcctc
      721 gtcaggaaaa ccgaaggcaa cattactgag caggtagaag atgcagtagc tcatagctcc
      781 ggactcagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
      841 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
      901 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      961 gttgttaact tgtttattgc agcttataat ggtt
//
