LOCUS       sequence-84928-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 549, Emission:
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-pBAD
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             148..165
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             169..201
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             214..234
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             241..951
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 actttgctat gccatagcat tttatccata gatagcggat actacctgac gcttttatcg
       61 caactctcta ctgtttctcc atacccgttt tttgggctag aaataatttg tttaacttaa
      121 gaaggagata tacatatgcg gggttctcat catcatcatc atcatggtat ggctagcatg
      181 actggtggac agcaaatggg tcgggatctg tacgagaacc tgtacttcca gggctcgagc
      241 atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag
      301 gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      361 cgcccctacg agggctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc
      421 ttcgcctggg acatcctgtc ccctcatttc acctacggct ccaaggccta cgtgaagcac
      481 cccgccgaca tccccgacta cttcaagctg tccttccccg agggcttcaa gtgggagcgc
      541 gtgatgaact acgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      601 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgtg
      661 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggt
      721 gccctgaagg gcaagatcaa gatgaggctg aagctgaagg acggcggcca ctacacctcc
      781 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc
      841 gacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      901 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agaattcgaa
      961 gcttggctgt tttggcggat gagagaagat tttcagcctg
//
